Transcript A | | b A

Nasonia vitripenni
You are the PI (Primary Investigator)
Jewel Wasp
Tell me about it…
Life Cycles
Haplo/Diplo Genetics
Wasp Development
Timelines
o
o
18 , 28 C
Complementation
Process by which an allele of each of two
mutant genes makes up for the defect in
the other gene.
You Find Three Mutants With a
Similar Phenotype
Are they the Same Genotype?
Possible Physiology
Enzyme A
Enzyme A
Enzyme A
Enzyme A
Enzyme B
Enzyme B
Enzyme B
Enzyme B
transport
Enzyme C
Enzyme C
Enzyme C
Enzyme C
Complement
a
a
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B
B
Think about dominant mutations!
x
a
A
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B
b
A
A
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b
b
Two genes
Both Recessive
Fail to Complement
A
A
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b
b
x
A
A
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b
b
A
A
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b
b
1 gene
Three scarlet eyed mutants, how many loci?
stDR, stC, st318
stDR
For example.
st318
Connect same
gene with a
line.
stC
Handling
Wasps
Sexing Wasps
adults
Sexing Wasps
larvae, tuxedo – black stages
Sex Pictures
Scoring Red Eyes
Here
Scoring Eye Color
Another Chart, Same Info
Wasp Business
Link
To Do
 Plan experiment!
Friday
 define symbols, diagram
crosses, determine
expectations, etc.
To Do
Friday -Wednesday?
…up to you!
 Each student:
complementation.
 Per bench, or per pair
(will tell next week),
 recessive, dominance,
 controls (stock
maintenance).
Plan Your
Crosses
Think about the wasp’s
schedule,
Think about your schedule,
Plan accordingly!
DR
C
318
st , st , st
F1 data, F2 data that means something
 Finish C. elegans experiment,
April 14 - 23
No Lecture April 13th
 Tinker with wasps,
 Exam Review (April 20th)
 Exam I (April 23rd),
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Basic Mendel!
Basic Mendel!
Basic Mendel!
Basic Mendel!
Basic Mendel!
C. elegans!
Bioinfomatics!
Nasonia!