Transcript Function of Sequence Elements (PowerPoint) Madison 2006

Group 2 - The Enhancers
Gene Expression
Unit Learning Goal
Identify the sequence elements of a gene and describe
their functions
A. Arrange the sequence elements of a gene in a
functional order
B. Predict the effect on gene expression if
components of functional genes are deleted,
inserted, or rearranged
C. Apply these concepts to a transgenic model
D. Integrate the functional relationships linking
protein, RNA, and DNA
Group 2 - Gene Expression
• Active Learning Activity
The exercise proposed is an in-class activity
utilizing “clickers”. This section will be
preceded and will compliment the gene
structure exercise elaborated by our group.
Students are encouraged to break into small
groups to discuss the questions posed to
them.
Group 2 - Gene Expression
• Introduction
Short lecture to introduce the idea of transgenics,
especially as it applies to the mouse system.
The following slides will introduce the GFP gene in
Jellyfish, its natural environment, and then we show
this gene being introduced into a mouse – transgene.
Jellyfish have the GFP gene which produces a “green
fluorescent protein”
Clicker Question 1:
Why is this mouse green?
a. It is producing the Green
Fluorescent Protein (GFP)
b. It is transgenic for the
GFP gene
c. It is expressing the GFP gene
d. All of the above
e. None of the above
Assume that
this mouse is
entirely green.
Discuss tissue specific expression of
the transgene.
Enhancer
Promoter
Structural gene
Reintroduce the modular nature of gene expression or gene
Regulation. Regulation can be separated from the structural gene
And that will emphasize where that protein is made is based on
the enhancer, and which proteins is made depends on the structural
Gene.
How do these two mice differ?
Clicker question 2:
Why are only their ears, tails and toes
green ?
a. Only the transgenic tissues
fluoresce
b. Jellyfish genes can only be
expressed in the skin of mice
c. Only skin cells contain the DNA
regulatory sequences for
expressing GFP
d. The GFP transgene linked to
mouse regulatory sequences
e. These mice all ate jellyfish
In order for the GFP gene to
function in the transgenic mouse?
a. the GFP gene must be similar in DNA
sequence to a mouse gene
b. the GFP protein must be similar in protein
sequence to a mouse protein
c. The GFP gene must be transcribed and
translated
d. All of the above
If the mouse has a jellyfish gene,
does that make it a jellyfish?
a. Yes
b. No