Transcript Slide 1 - Springer Static Content Server

Supplementary figure 1
Supplementary figure 1. The box-and-whisker plot to examine the distribution of
feature intensities.
The x-axis represents the individual microarray, while the y-axis represents the
feature intensity values. Boxes represent the interquartile range, with the 75th
percentile at the top and the 25th percentile at the bottom. The line in the middle of the
box represents the 50th percentile, or median, while the plus represents the mean.
Whiskers represent the rest of the distribution, with their terminations representing
the lowest and highest feature intensity values.
Supplementary figure 2
Supplementary figure 2. Ingenuity pathways analysis: Network of down-regulated
genes in response to 1µg/ml mCRP
Networks of gene/gene product interaction were generated using IPA (Ingenuity®
Systems, www.ingenuity.com). Genes or gene products are represented as nodes, and
the biological relationship between two nodes is represented as an edge (line). All
edges are supported by at least one published reference. Solid edges represent a
direct relationship and dashed edges represent an indirect relationship. The green
node color represents down-regulation in response to mCRP. The shape of each node
represents the functional class of the gene product, as shown in the legend of figure 4.
Supplementary figure 3
Supplementary figure 3. Ingenuity pathways analysis: Network of down-regulated
genes in response to 5µg/ml pCRP
Networks of gene/gene product interaction were generated using IPA (Ingenuity®
Systems, www.ingenuity.com). Genes or gene products are represented as nodes, and
the biological relationship between two nodes is represented as an edge (line). All
edges are supported by at least one published reference. Solid edges represent a
direct relationship and dashed edges represent an indirect relationship. The green
node color represents down-regulation in response to pCRP. The shape of each node
represents the functional class of the gene product, as shown in the legend of figure 4.
Supplementary figure 4
WB: anti MX1
mCRP
pCRP
INFα2A
ladder
50 kD
Supplementary figure 4. MX1 expression detected by Western blot from
lysates of mCRP (1g/ml), pCRP (5g/ml), or IFNa2A (1 ng/ml) treated EPCs
Representative Western blot of MX1 (arrow) detected by a polyclonal rabbitanti-human antibody.
Supplementary figure 5
Quantitative PCR
of selected genes after IFNα2A treatment
OAS3
IFI6
IFI44
IFI27
OAS2
MX1
IFI44L
0
100
200
300
400
500
600
700
800
fold change in gene expression
Supplementary figure 5. Quantitative real-time PCR of selected genes after IFNa2A
(1 ng/ml) treatment of EPCs.
The expression of selected genes that were found to be highly upregulated in
response to mCRP treatment was quantified in EPCs following treatment with
IFNa2A. The mean in gene expression was obtained using differences in cycle
threshold between the gene and 18s (Ct). The fold change in difference (Ct) in
gene expression in the IFNa2A treated samples compared to the PBS control
samples was determined (2Ct) and expressed in the diagram as fold change in
gene expression.