Transcript HGP

```I. Observation or Introduction to the Problem
II. History of Field or Background Information
III. Hypothesis or Objectives
IV. Methods of Study
a. Techniques
b. Experimental Design or Protocol
V. Results (4-6 slides)
VI. Summary (state the findings)
VII. Conclusions, Speculations and Plans
CHECK OUT THE ROOM
SIZE OF ROOM
PODIUM
POINTER
COMPUTER HOOKUP
MICROPHONE
SCREEN
• felt vs thought or believe
• incidence vs prevalence
• phenomenon(na)
• parameter vs variable
• constitute vs comprise (consist)
• effects vs affects
#1 Mistake in Science Talks:
The Assumption That the Audience Knows
Keep it Simple: Explain!
Introduction- Observation
Keep it simple: engage the audience
Sets up the rationale for study
Abstract talks: the first slide reflects title
Long talks: Historical basis of problem
Current understanding
Background information
Must have one and be able to state it
•Make no assumptions
•Be specific
Tailor description to audience
Simplify
Explain methods
Explain abbreviations and jargon
Time line must be clear
Methods
In order to measure isoprostanes, we first collected pig urine
Figures and Data Slides
Declarative title
Point to axes-describe
1 min per slide
Speak to the slide
Don’t over-interpret data
Avoid tables!
Common Errors in Data
Display and Graphing
Indexing/Proportionality
Scaling
Complexity
Credibility
Summary
After careful study, most investigators have
concluded that tables generally stink as a way
to portray information to an audience.
Conclusion
If you want to present a good talk, avoid tables.
Proportionality Problems: What is a Doubling?
Radius = 1 Area = r2 Volume = r3
r=2
Area = 4
Volume = 8
r=1
Area = 1
Volume = 3
r = 1.26
Area = 1.6
Volume = 2
Pa Pressure Doubled After Endotoxin
100
…. ………….
SBP
mmHg
50
….
…
……….
0
E
TIME
Ppa
Ppa Doubles After Endotoxin
30
Ppa 20
10
0
E
TIME
X Increased after Treatment
100
X
0
…
..
..
...
..
.
..
.…
PRE
..….
..
..…
…
…
..
..
..
.
.
POST
X Increased after Treatment
100
.
X
.
0
PRE
POST
Substance P is a Potent Pressor
80
mmHg
70
PRE
POST
Substance P is a Mild Pressor
100
mmHg
0
PRE
POST
TGFb: Involvement in PPH Pathogenesis
•Cell proliferation,
differentiation
•Apoptosis
•Morphogenesis
•Organogenesis
Linkage & Disease Gene Hunting 2000+
Family
Studies
Chromosome
Interval
Candidate
Genes
Disease
Mutation
Met A
T
G
Val G
T
C
Ser T
C
A
Leu C
T
G
Gln C
A
A
Pro C
C
G
Cys T
G
T
Genes in Interval
*
Genetic
Mapping
Computer
Search
1.
ESTs, unidentied
2.
ESTs, unidentied
3.
ESTs, highly similar to
patched [Drosophila
melanogaster]
4.
Phosphofructokinase (PFK)
5.
BMPR2 Positional &
Functional candidate for
PPH
6.
ESTs, unidentied
7.
Deleted in pancreatic cancer 1
(DPC1)
8.
ESTs, unidentied
HGP
Mutation
Detection
A
T
G
G
T
C
T
C
A
C
T
G
T
A
A
C
C
G
T
G
T
Met
Val
Ser
Leu
STOP
Summary: be concise
statement of facts
Conclusions: meaning or lesson
speculations
Problems and Plans: what is next
what to fix
Oh, yes: finally
• you probably shouldn’t make every
slide a different font and color
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