Transcript Newdesign2003

Heat shock pulse
T7 polymerase
Very few molecules of T7 polymerase are produced by heat shock pulse.
Supposedly only one molecule binds to one side of the two partially
OVERLAPPING T7 promoters and activate the downstream gene. At least
two genes(actually an AD also need to be expressed if combined with the
memorizer) are expressed: one repressor for the other side so that only
the side hit by the polymerase can fire again, and one T7 polymerase to
sustain the gene expression, stabilizing the state of the Randomizer.