Transcript navon

Exploring a Putative
Gene
‫גיא וקסמן‬
‫רועי נבון‬
Problem definition
Taking one of the thousands of
Putative genes that were “found”
In the human genome project &
Trying to determine whether this
Sequence might be a real gene that can
be translated into a protein product.
The Selected Gene:
NM_014727
Homo sapiens myeloid/lymphoid
or mixed-lineage leukemia 4
(MLL4).
PSI-BLAST
The Locus problem
Many entrances are actually on the
same locus (SNPs, flanking, different
Alleles etc`).
Different Entrances on the Same
Chromosomal Region
Conserved Domains
CXXC zinc finger
Zinc finger
SET1
2-BLAST
2-BLAST between the putative MLL4
gene & the most similar real GeneTrithorax, from Drosophila
Melanogaster.
E=5e-87
E=2e-82
Multiple Alignment
SET1 domain
3D Structure –
Zinc Finger Domain
Biological Aspects
Drosophila melanogaster
The trithorax gene, a trans-acting regulator of the bithorax complex
in Drosophila, encodes a protein with zinc-binding domains.
Mazo AM, Huang DH, Mozer BA, Dawid IB
‫ככה נראיתי‬
...‫פעם‬
Homo Sapians
Acute mixed-lineage leukemia t(4;11)(q21;q23) generates
an MLL-AF4 fusion product
Conclusions
1. We found a high similarity between our
putative gene and genes from other
species.
2. The MLL4 putative gene contains at least 3
conserved domains.
3. The homologue genes from Drosophila and
Human are associated with the nervous
system and leukemia respectively.
Possible directions for
future research
1. Trying to find DNA motifs which bind to our
putative gene and to its homologues.
2. Trying to isolate the full length protein.
3. When 3D structure prediction will be
available, we’ll try to compare our putative
protein with other known proteins.