Transcript Document
Central dogma • DNA is made (transcribed) into RNA • RNA is made (translated) into protein DNA is made into RNA • A protein enzyme, polymerase, transcribes (makes) DNA into RNA. You will transcribe DNA into RNA today! RNA made into protein RNA is genetic material that codes for protein RNA must contain the correct code to make the correct protein Ribozyme • RNA that acts as an enzyme • Enzymes speed up a reaction • Ribozymes cleave (cut) RNA Splicing Splice – to join together at the ends Genes can be cut and pasted together (spliced!) Why Splice? Exon 1 Intron Exon 2 Certain parts of RNA do not code for proteins, they need to be cut out Exon – part of gene that codes for protein Intron – does not code for protein, splice it out! Splicing Before splicing Exon 1 Intron Exon 2 After splicing Exon 1 Exon 2 Exon –codes for protein Intron – does not code for protein, splice it out! Splicing Before splicing Exon 1 Intron Exon 2 After splicing Exon 1 Exon 2 Exon –codes for protein Intron – does not code for protein, splice it out! Ready for Protein! Before splicing Not ready to be made into protein After splicing Ready to be made into protein Exon –codes for protein Intron – does not code for protein, splice it out! Ribozyme • Ribozymes can splice RNA together You will be splicing RNA together using a ribozyme today What will you do? • You will transcribe DNA into RNA. This will be done by incubating the DNA with polymerase, the enzyme that turns DNA into RNA. What will you do? • The RNA that you will make contains a ribozyme. The ribozyme will splice itself! Each group will transcribe and splice for a different amount of time. Group 1- 5 minutes Group 2 – 10 minutes Group 3 – 15 minutes Group 4 – 20 minutes What will it look like?