Transcript Using BLAST and ClustalW2 for Multiple Sequence Alignment

Using BLAST and ClustalW2 for
Multiple Sequence Alignment
IBGP/BMI705 Lab 2
January 14, 2009
Lee Cooper, TA
[email protected]
Have you ever used
bioinformatics tools?
Why should I care about
understanding bioinformatics?
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Applicable
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Free, readily-available tools
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Focus benchwork
Preliminary data
vast amounts of information
powerful
Required knowledge in 5-10 years
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Career marketability
Summary: BLAST/ Sequence
Alignment
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A—Search genes (5 minutes)
B—BLAST (10 minutes)
C—Pair BLAST (10 minutes)
D—ClustalW2 (10 minutes)
Today’s guided lab: BLAST/
Sequence Alignment
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Follow handout
Ask questions
Work together
Answer questions
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short-answer format on paper
turn in to TA today (Pass/fail)
Next week’s lab– SAME handout and tools
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Practice outside of class
How can you use these tools to help along your
current research and/or studies?
A. Search for gene/s
A. Search for gene/s
A. Search for gene/s
A. Search for gene/s
A. Search for gene/s
B. BLAST
B. BLAST
B. BLAST
C. Pair BLAST: Comparing Homo
sapiens E2F3 to Mus musculus E2f3
C. Pair BLAST: Comparing Homo
sapiens E2F3 to Mus musculus E2f3
D. ClustalW2 Sequence Alignment
D. ClustalW2 Sequence Alignment