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Lab Instructor Name: Quiz 12: Biol 203 2013 Name:_______________ Last core biology class (Biol102, 202, 300) taken at Hunter?_______ and grade________ (1pt) Grade you expect in this class?__________ (1pt) EACH QUESTION IS WORHT 1PT AND IS ALL OR NONE. 1) The following genomic sequence represents a TOP strand: 5’AAATTGATCGATCCCC 3’ Write out the antiparallel strand in the 5’ to 3’ orientation:_________________ 2) Name the 4 most common RNA bases (spell out) ___________ _____________ ______________ ___________ 3) A fifth common RNA base ________ is used in tRNA for wobble. 4) Name the 5 most common DNA bases (spell out)__________ __________ __________ _________ _________ 5) cDNA is made from mRNA by the enzyme______________________ 6a) Double stranded cDNA for a protein coding gene usually has what three identifiable regions: ___________ ___________ _____________ 6b) Must these three regions equate to three exons? (yes, no –circle one) 7) The triplet code for translation contains how many different triplets recognizable by tRNAs?____ 8) PCR is an abbreviation for________________________? 9) Why is 15 bases the minimum for a oligo nucelotide design during a PCR experiment?________________________ 10) SNP is an abbreviation for______________________________? 11) Given random DNA , how frequent will the sequence TATA be found in the genome?________ 12) What three regions (not counting SD and SA sites) of a gene are not found in the mRNA?________ ________ _______ 13) What two regions of a mRNA are not found in the gene?____________ ____________ 14) What portion of a cDNA (encoding a protein) is usually conserved between species?___________ 15a) What word describes a completely non-functional allele?______________ 15b) What type of a mutation is most likely to create such an allele?____________ 16) ORF is an abbreviation for ________________________. 17) CDS is an abbreviation for ________________________. 18a) Can all CDS’ be said to contain an ORF? (yes, no-circle one) 18b) Can all ORFs be said to contain a CDS? (yes, no-circle one) Quiz 12: Biol 203 2013 Lab Instructor Name: Name:_______________ Using the following 11 pieces only, design a transgene and a transcription factor target gene that could be deleted within any cell in such a way to create a neomorphic allele using Tamoxifen induction. All splice forms of the transcription factor lead to functional proteins. You can assume SD, SA and pA sites are not listed and will be found in the appropriate places in the two genes . R A CreERt2 Transgene Transcription factor CMV HD 11pts, Each piece incorrectly placed on the line below will receive a 1 pt deduction. Using the following pieces only, design a transgene and a transcription factor target gene that could be deleted in such a way that a neomorphic allele would be created. All splice forms of the transcription factor lead to functional proteins. You can assume SD, SA and pA sites will be found in the appropriate places CreERt2 CMV Transcription factor A R HD All exons contain TM domains (7 total) , 1st domain is extracellular