Transcript Document

An RFLP was found to be associated with the X-linked recessive disorder hemophilia A in a particular family. A pedigree and
Southern blot analysis are shown below. Individual II-1 is affected. Assuming that no recombination has occurred, what is the status
of the individuals in generation III with respect to hemophilia A?
a. III-1 and III-3 are affected, III-2 is normal, and III-4 is a carrier.
b. III-1 and III-3 are affected, III-2 is normal, and III-4 is not a carrier.
c. III-1 and III-3 are normal, III-2 is affected, and III-4 is a carrier.
d. III-1, III-2, and III-3 are affected, and III-4 is a carrier.
e. All individuals in generation III are normal.
1
1. A researcher identified a new enzyme and is interested in determining the mRNA expression pattern of this gene. Which of the following
techniques would accomplish this goal? A. Southern blot B. northern blot C. western blot D. eastern blot
2. You are hired as a scientific consultant for a popular television show about forensic analysis of crime scene evidence. You are told that in an
upcoming scene, investigators will compare the DNA profiles of a known suspect and an unknown biological sample collected from the
victim. You are asked to write up a brief explanation of the primary technique required for discussion in the scene. Which technique should
you write about? A. DNA fingerprinting B. DNA footprinting C. RFLP analysis D. PCR
3. Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another,
using molecular cloning techniques? A. restriction fragment length polymorphism data B. sequence analysis C. Southern blot
data D. restriction maps of the plasmids
4 Which of the following two enzymes are needed to construct a cDNA library? A. reverse transcriptase and DNA ligase B. reverse
transcriptase and Taq polymerase C. DNA ligase and Taq polymerase D. Taq polymerase and RNA polymerase
5. In a manual enzymatic DNA sequencing reaction, the nucleotide ddT is incorporated A. at every T position in the synthesized DNA strand;
the reaction is terminated when all T positions have been filled with ddT. B. randomly at a single T position in the synthesized DNA strand,
leading to termination of the reaction. C. at several T positions in the synthesized strand, leading to termination of the reaction. D. randomly
at a A position in the template DNA strand, leading to termination of the reaction.
2