Transcript 13.4_Gene_Regulation_and_Expression

1 Review What genes control cell differentiation
during development
Compare and Contrast How is the way Hox genes
are expressed in mice similar and different to the
way they are expressed in fruit flies
2 Use Analogies Write an analogy that demonstrates
how the lac repressor functions
3 A hormone is a chemical that is produced in one
part of the body, travels through the blood, and
affects another part of the body. Many hormones
are proteins. How might the production of a
hormone affect the expression of genes in a
eukaryotic cell? Write a hypothesis that could be
tested to answer this question (include promoters
in your hypothesis)
CH 13 RNA AND PROTEIN SYNTHESIS
13.4 Gene Regulation and Expression
Prokaryotic Gene Regulation

Bacteria and other prokaryotes do not need to
transcribe all of their genes at the same time
 Saves
time and resources
 Regulate with DNA binding proteins
 Turn genes “on” or “off.”
Operons
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Group of genes that are regulated together
Genes usually have related functions
E. coli have cluster of 3 genes that must be turned
on before it can use lactose as food
 Lac
operon.
Promoters and Operators

Promoter (P)
 Site
where RNA-polymerase can bind to begin
transcription

Operator (O)
 Where
the DNA-binding protein, lac repressor, can
bind to DNA.
Lac Operon “Off”

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
No lactose present
Lac repressor binds to the O region
RNA polymerase is blocked.
Lac Operon “On”




Lactose present
Attaches to the lac repressor and changes the
shape
New shape makes it fall off the operator
RNA polymerase can bind and transcribe.
Eukaryotic Gene Regulation

TATA box
 Contains

the sequence TATATA or TATAAA
Binds a protein that helps position RNA
polymerase.
Transcription Factors



DNA-binding proteins
Enhance transcription by opening up tightly packed
chromatin, attract RNA polymerase, or block
access to certain genes
Attach to RNA polymerase before it binds to DNA.
Cell Specialization


Many eukaryotes are multicellular
Different cells have very different job but yet all
have the same DNA.
RNA Interference

Small RNA molecules
 Not




mRNA, rRNA, or tRNA
Get transcribed
Fold over to become double stranded
Enzyme called the “Dicer” enzyme cuts, or dices,
these double-stranded loops into microRNA
(miRNA)
Two strands separate.
RNA Interference


miRNA pieces attaches to a cluster of proteins
Forms a silencing complex.
RNA Interference


Silencing complex binds to complementary mRNA
miRNA sticks to mRNA and stops their proteinmaking instructions.
RNA interference (RNAi)

Blocking gene expression by means of an miRNA
silencing complex.
The Promise of RNAi Technology


Ability to turn on or off genes
Work with viruses or cure cancer?
Genetic Control of Development
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As an embryo develops, different sets of genes are
regulated by transcription factors and repressors
Gene regulation helps cells undergo specialization.
Homeotic Genes


Master control genes that regulate organs that
develop in specific parts of the body
Homeobox
 Group
of the same homeotic genes
 Important in cell development and differentiation.
Hox genes


Determine the identities of
each segment of the body
Arranged in the exact order
in which they are
expressed, from anterior to
posterior.


Hox genes exist in the DNA of
other animals
Tell the cells of the body how
to differentiate as the body
grows.
Environmental Influences

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Presence of lactose in E. coli
Bullfrog metamorphosis depends on conditions - a
drying pond, a high density of predators, low
amounts of food- tadpoles may speed up their
metamorphosis.