Transcript AIMS OF RESEARCH – Model plant Arabidopsis - bioemit

Metabolic profiling of Arabidopsis thaliana
::
post-doc Jens Rohloff
Working Plans & Goals - Project year 2004
AIMS OF RESEARCH – Model plant Arabidopsis thaliana

Characterize secondary metabolites in At with focus on
the glucosinolate-myrosinase complex
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Investigate T-DNA-knockout mutants being studied within The Plant
Genetics Group at NTNU to sustain the group’s molecularbiological work

Study both abiotic and biotic factors affecting plant growth and metabolism
regarding morphology and ontogenetic development
Metabolic profiling of Arabidopsis thaliana
::
post-doc Jens Rohloff
The glucosinolate-myrosinase complex
Glucosinolates derived from amino acids
- leucine
- methionine
- phenylalanine
- tryptophane

indole glucosinolates
Altered pathway of GLS induces
changes in biosynthesis of other
secondary metabolites involved in
- plant defense
- plant-insect interactions
- stress response
Detection of concentration levels of
- Glucosinolates
- Plant hormones
- Amino acids
- Volatiles, i.e. isothiocyanates,
nitriles, terpenes, hydrocarbons
Metabolic profiling of Arabidopsis thaliana
::
post-doc Jens Rohloff
Preliminary Results 2003 - Volatiles from Arabidopsis
Metabolic Profiles from A. Arabidopsis mutants (wt,TGG1,
TGG2,Cyp83A1,Cyp83B1)
B. Ecotypes of Arabidopsis
(Col, Cve, Ler, Was)
C. Plant organs
(leaves, stems and flowers)
D. Living plants (in vivo) or
Autolysed plants
Identified Volatiles
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Terpenes
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Green leaf volatiles
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Isothiocyanates + Nitriles
 Hydrocarbons (alkanes, aldehydes
ketones,esters)
::
post-doc Jens Rohloff
Gas chromatography analysis (GC-MS) of
volatiles from Arabidopsis mutant Cyp83A1
tetradecanal
2-ethyl benzyl ITC
nonyl ITC
Method - headspace solid-phase microextraction
hexyl ITC
decanal
heptyl ITC
4-methyl pentyl ITC
3-methyl butyl ITC
nonanal
para-cymene
Z-3-hexenyl acetate
alpha-phellandrene
1-hexanol
Z-3-hexenol
Metabolic profiling of Arabidopsis thaliana
. . . over 100 chemical compounds have been tentatively identified
by mass spectral database search (GC-MS) !
Metabolic profiling of Arabidopsis thaliana
::
post-doc Jens Rohloff
Co-operation within BIOEMIT and outside the project
1. The Plant Genetics Group, Dep Biol, NTNU
Ishita Ahuja (res.) + Anna Kusnierczyk (dr.stip.)
Anna Vera De Felice (post-doc)
 plant-insect interactions
 metabolomics
2. Institute of Physical Chemistry, Dep Chemistry, NTNU
Prof. Bjørn Alsberg, Endre Anderssen (post-doc)
 bioinformatics
3. The Plant Biocentre, Dep Biol, NTNU
Prof. Tor-Henning Iversen, Knut Robert Fossum (res.)
 space biology
4. Neuroscience Lab, MTFS, NTNU
Stig Ulland (dr.stip.)
 plant-insect interactions
5. Group of Ecological Chemistry, Dep Org Chemistry, KTH
Prof. Anna-Karin Borg Karlson
 biochemistry
Metabolic profiling of Arabidopsis thaliana
::
post-doc Jens Rohloff
First publication title (submission to Phytochemistry until April 2004)
“Altered headspace volatile profiles of Arabidopsis mutants
with deficiencies in the glucosinolate biosynthetic pathway”
Conference
15. Int Conf Arabidopsis Research, July 10 - 14, 2004, Berlin
Fellowship abroad (3 up to 6 months in 2005)
Max-Planck-Institute at Jena or Potsdam, Germany
>>> funding will be applied via EU (Marie Curie), NFR or DAAD