Transcript control At2g44130::LUX +CJ - Springer Static Content Server

Fig. S 1
a
b
CJ-induced genes classed by ontology. Distribution of CJ induced genes, obtained by cDNA microarray (a)
and ATH1 oligonucleotide microarray (b) analysis, into functional categories. Genes were assigned to
various functional categories based on searching the TAIR or MIPS databases for suggested GO
annotations.
Fig. S 2
a
b
At2g44130::LUX
control
+CJ
The promoter of the F-box At3g28740 was translationally fused to luciferase and stably introduced
into Arabidopsis. Expression was induced by the exogenous cis-jasmone vapours (b) but not in the
control (a). Luciferase activity was detected after application of luciferin and imaging of whole plants
(rossette stage) via CCD. Expression was detected predominantly in the midrib of leaves.
Fig. S 3
a
b
YAACKG (myb2 motif)
TGAC
a
a
b
a
a
bb
RCCGAC
(DRE/CRT core motif)
TGACGT
a
a
cis-jasmone inducible promoters at 3 hpt (affymetrix array)
cis-jasmone inducible promoters at 20 hpt (cDNA array)
a
b
YACGTGGC
(ABRE core motif)
WRKY motif : TAGWCT
(van der Ent et al., 2009)
a
a a
a
background (random Arabidopsis promoters)
Fig. S3: Occurrences of cis-acting elements in the promoter regions of CJ-inducible
genes. motifs were quantified in the 500-bp sequences preceding the 5'-end of each
transcription unit according to POBO bootstrapping analysis (Kankainen & Holm, 2004b).
Different letters indicate statistically significant differences in occurrences of ciselements between promoter groups (χ2 test; α=0.05).
a Occurrences of as-1 core motif TGAC in CJ-inducible genes at 3h and 20h of exposure
to CJ in comparison to randomly selected promoter sequences from the Arabidopsis
genome.
b Occurrences of core sequences of different stress-related cis elements in the
combined set of CJ-inducible genes at 3h and 20h of exposure in to randomly
selected promoter sequences .
YACGTGGC
(MYB core motif)
a
a
cis-jasmone inducible promoters at
3 and/or 20 hpt
background (random
Arabidopsis promoters)
Fig. S 4
Bla-14
Sha
Li-3
In-0
Gr-3
Go-2
Fr-4
Cvi-0
Bu-6
Ler
Col-0
a
Col-0
All samples + CJ
At2g44130
TGA-independent
Bla-14
Sha
Li-3
In-0
Gr-3
Go-2
Fr-4
Cvi-0
Bu-6
Ler
Col-0
b
Col-0
RNA loading
At3g28740
TGA-dependent
At4g34135
RNA loading
Fig. S4. Analysis of cis-jasmone response of twelve different Arabidopsis ecotypes by Northern blot. Four plants
per ecotype (4 weeks old) were exposed for 20 hrs to cis-jasmone, harvested, the leaves pooled and RNA
extracted. Seven micrograms of total RNA was loaded per lane and the blot was hybridised with the probes as
specified, representing the TGA-independent pathway (a) or TGA-dependent pathway (b) as described in Fig. 6b.