Transcript -Pregnancy test Urine (EVA test) -The Measurement of Urinary 17

-Pregnancy test Urine (EVA test)
-The Measurement of Urinary 17Oxosteroids.
376 BCH
Miss Tahani Al-Shehri
Pregnancy test
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HCG- Human chorionic gonadotropin , Mw 36,700 ,
chemically a glycoprotein , 30% CHO and amino acid
Normally person not pregnant has a secretion of this
hormones but not detected
Function of HCG
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Stimulate the biosynthesis of ovarian tissue
Convert androgen (men) to estrogen (women ) on placenta
Maintain pregnancy
Pregnancy test
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It can give +ve result in these case :
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Hyperactivity of pituitary gland
Functional abnormalities of placenta .
Intrauterine fetal death .
The Measurement of Urinary 17Oxosteroids.
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OBJECTIVES
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To measure the excretion of 17-oxosteroids in a 24hour collection of urine.
INTRODUCTION AND PRINCIPLE
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Most of the products and metabolites of the androgen pathways in
the adrenal and the testis, are in the form of 17-oxosteroids.
The quantity of 17-oxosteroids (17-OS) excreted varies with
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In females, most of the 17-OS are derived from the
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adrenal gland
In the male a considerable percentage (10-20%) is derived from the
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Age
Sex
weight.
testis as metabolites of testosterone (not itself a 17-OS).
The adrenal cortex and testis are both under the hormonal control
of the anterior pituitary.
Thus, changes in the 17-OS excretion in urine are found in disease
involving the anterior pituitary, adrenal cortex and testis.
INTRODUCTION AND PRINCIPLE
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Decreased excretion of 17-OS is found when there is
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Increased excretion of 17-OS in both sexes is normally
associated with
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hypo-activity of the adrenal cortex, pituitary, or testis.
increased activity of the adrenal cortex
High levels of 17-OS may be found in some tumors of the
testis or ovaries.
Steroids are normally excreted as water-soluble
conjugates, glucuronides, and sulphates, since the steroids
themselves have very low solubilities in water.
INTRODUCTION AND PRINCIPLE
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These conjugates must be hydrolyzed to liberate the
free steroid, before extraction from the urine into an
organic solvent, usually chloroform or ethylene
dichloride.
The extract is then washed to remove impurities,
which interfere with the colorimetric assay and the
oxo-steroids are estimated using Zimmerman's
reagent.
Zimmerman's reagent is an alkaline solution of ndinitro-benzene, which slowly forms colored
complexes with steroids containing CO.CH2 groups.