Transcript PFK1

PFK through the ages
Jonathan A. Atwater
Janet A. Baker
Chitra Chandrasekaran
Lizette Santos-Santori
Project goal
The primary goal of this project is to put
metabolism in an evolutionary
perspective by analyzing the similarities
and differences among taxonomic
groups for ATP-dependent
Project audience
The project will be directed to
undergraduate students in the following
General Biology
 Cell Biology
 Biochemistry
Project materials
base file of amino acid sequences for
phosphofructokinase for the following organisms:
Clostridium tetani
Bacillus cereus
Anabaena variabilis
Bateriodes fragilis
Thermus aquaticus
Uncultured archaea
Thermoproteus tenax
Arabadopsis thaliana
Sacchromyces cerevisiae
Mus musculus
Homo sapiens
Pan troglodytes
Xenopus laevis
Danio rerio
Drosophila melanogaster
Caenorhabditis elegans
General Objectives
Problem-based approach to examining
protein structure and function from an
evolutionary perspective
 Using the evolutionary perspective to
provide a unified approach to link the
study of protein structure and function to
Specific objectives
Analyze amino acid sequence for each
 Perform a multiple sequence alignment:
 Use the alignment to identify important
functional domains.
 Infer phylogenetic relationships.
Future plans
Include more organisms in the study.
 Use amino acid sequence alignment to
examine protein structure at a three
dimensional level (Protein Explorer).
 Examine the evolution of active sites and
allosteric regulatory sites of
 Establish evolutionary connections by
phylogenetic analyses.
Resources/references (NCBI)
 (BioQuest)
 (Biology
m (Protein Explorer)