Transcript PFK1

PFK through the ages
Jonathan A. Atwater
Janet A. Baker
Chitra Chandrasekaran
Lizette Santos-Santori
Project goal
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The primary goal of this project is to put
metabolism in an evolutionary
perspective by analyzing the similarities
and differences among taxonomic
groups for ATP-dependent
phosphofructokinase.
Project audience
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The project will be directed to
undergraduate students in the following
courses:
General Biology
 Cell Biology
 Biochemistry
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Project materials
Data
base file of amino acid sequences for
phosphofructokinase for the following organisms:
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Clostridium tetani
Bacillus cereus
Anabaena variabilis
Bateriodes fragilis
Thermus aquaticus
Uncultured archaea
Thermoproteus tenax
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Arabadopsis thaliana
Sacchromyces cerevisiae
Mus musculus
Homo sapiens
Pan troglodytes
Xenopus laevis
Danio rerio
Drosophila melanogaster
Caenorhabditis elegans
General Objectives
Problem-based approach to examining
protein structure and function from an
evolutionary perspective
 Using the evolutionary perspective to
provide a unified approach to link the
study of protein structure and function to
metabolism
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Specific objectives
Analyze amino acid sequence for each
organism.
 Perform a multiple sequence alignment:
CLUSTALW.
 Use the alignment to identify important
functional domains.
 Infer phylogenetic relationships.
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Future plans
Include more organisms in the study.
 Use amino acid sequence alignment to
examine protein structure at a three
dimensional level (Protein Explorer).
 Examine the evolution of active sites and
allosteric regulatory sites of
phosphofructokinase
 Establish evolutionary connections by
phylogenetic analyses.
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Resources/references
www.ncbi.nlm.nih.gov (NCBI)
 www.bioquest.org/bedrock (BioQuest)
 http://workbench.sdsc.edu (Biology
Workbench)
 http://molvis.sdsc.edu/protexpl/frntdoor.ht
m (Protein Explorer)
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