Transcript 7.391_sample

Characterizing Novel Aquatic
Phages from the Environment
7.391
Minyoung Jang
January 19, 2007
Overview
• Take water sample from the environment
• Isolate: column, extract protein (CsCl),
DNA (phenol chloroform)
• Characterize: titer, identify hosts that can
be infected, PCR, sequencing, digests
SDS-PAGE
• Denature isolated, purified proteins by
SDS + boiling
• Acrylamide gel
• Load known samples & molecular weight
(MW) markers
Our results
• Gel image here– LARGE,
easy to see
• Label all lanes
• Label MW markers
• Label important bands
• Describe conditions: time
run, voltage, & anything
non-standard, how much
you loaded
• ALL LABELS SHOULD
BE EASY TO SEE!
• Describe interesting
results VERBALLY (not
written)
– How many different distinct
bands do you see? Any
particularly strong? What
weight?
– Do any bands overlap with
your “host cell” sample
bands (ie those bands are
not from phage)
– Etc.
– Be ready to answer
questions about your
protocol & data
Bioinformatics Homework
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Protein identified
Source organism
Protein function
Amino Acid length
Known structures, family of proteins
Other functions of homologs
LIST ALL SOURCES!! (can footnote it &
abbreviate [1]
[1] Weigele, et al., 2007. Journ. Name Abbrev. Form.