Transcript Amylase Lab

Word of the Day:
Enzyme:
An enzyme is a substance that
starts a chemical reaction in
cells
Enzyme
Chemical acted
upon
Product
lipase
Fats and oils
Glycerol and fatty
acids
Protease
Proteins
Amino acids
Catalase
Hydrogen
peroxide
Water and oxygen
Amylase Lab
What does your spit do to food?
Background
• Amylase is an enzyme found in your
saliva
• Amylase breaks down starches and
turns them into sugars
Remember your Latin and Greek
• Starch -------------> glucose + glucose + glucose +…
Substrate
Type of
starch
Amylase
enzyme
Product
Found in
saliva
Type of sugar
which your body
uses for energy
Let’s see how it really works…
• Step 1: Preparing the Lab
– Get 10 test tubes
– Label 1-10
– Add 5 drops of
iodine to each test tube
2 dropper
fulls of
iodine
1
1
Step 2: Prepare the saliva
solution
• Collect 4 ml of saliva – Spit through the straw into a
clean graduated cylinder
– Add 17 ml of
water to graduated
cylinder and mix.
Use straw
to collect
saliva
Step 3: Prepare the Starch
Solution
• Using a 2nd graduated cylinder, get 7ml
of starch solution and pour into a small
beaker
Measure 7ml of starch
solution into beaker
Step 4:
• Mix the saliva solution into the starch
solution
• Record the exact time of mixing
Saliva
solution
Starch solution
Step 5: Add Saliva solution to
Test Tube #1
• Add 10 drops of saliva/starch to test
tube # 1
• Record color:
– Black/Purple: starch present
– Yellow/Brown: no starch present
Step 6: Repeat Step 5, Every
Three Minutes
• Every three minutes, add 10 drops of
saliva-starch solution to a new test tube
– Record color
– Repeat until the starch is no longer present
Data Table
Time
0
3
6
9
12
15
18
Starch Present (Yes/No)
Claim, Evidence and
Explanation
• Write a CEE
• Due Next Class