Transcript The Play is the thing…

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Transcription and Translation
Objectives
• How ‘we’ go from DNA to protein
• Similarities and differences between
nucleic acids and amino acids
• Work through transcription and
translation
• Transcription: writing again
• Translation: changing languages
Today
we’ll go
from
here...
Text
To here
Sending ‘messages’ out from DNA
• DNA replication
– both strands => new
DNA
– => new cell
• Transcription
– 1 strand => new RNA
– => new protein
Amino Acids
• How are they similar?
• How are they different?
• What do the differences mean
in terms of “feel”?
• How many are there?
• Possible?
• Check ‘em out on homepage
Amino Acids
• You & partner (s) have an
amino acid; which is it?
(StructViewer or homepage =>
left column ‘big twenty’ amino
acids)
vs. Nucleic Acids?
• How are they similar?
• How are they different?
• What do the differences mean in terms
of “feel”?
• Which is more diverse in terms of
shape and ‘feel’?
• Which would allow for more diverse
shapes and surfaces when
‘connected’?
A little Vocab
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DNA
mRNA
rRNA
tRNA
Codon
Anticodon
How does a codon ‘mean’
an amino acid?
The Play is the thing…
The Play is the thing…
Or, Fun With Blocks
The Play is the thing…
• ‘Types of Bonds’
– Velcro – can be easily broken/re-made
during lab
– Duct tape – breaking it gets you a zero (0)
for this week’s quiz
The Play is the thing…
• The Players
The Play is the thing…
• The Players
– tRNA
The Play is the thing…
• The Players
– tRNA
– Ribosome
The Play is the thing…
• The Players
– tRNA
– Ribosome
– Aminoacyl tRNA synthetase
The Play is the thing…
• The Players
– tRNA
– Ribosome
– Aminoacyl tRNA synthetase
– RNA polymerase and mRNA
The Play is the thing…
• The Players
– tRNA
– Ribosome
– Aminoacyl tRNA synthetase
– RNA polymerase and mRNA
– Termination factor
The Play is the thing…
• The Players
– tRNA (4 people)
– Ribosome (1)
– Aminoacyl tRNA synthetase (4)
– RNA polymerase and mRNA (1-2) *
– Termination factor (1)
DNA Template
• 5’ CTTAAATCCGAATGCCCATG 3’
5’ is “sticky”, 3’ is “fuzzy”
5’ CTTAAATCCGAATGCCCATG 3’
5’ is “sticky”, 3’ is “fuzzy”
Learning your ‘lines’
• Handout: Each group find questions
related to your role; answer them
• Lab manual, textbook, internet OK as
sources
• Meet your blocks-- 5’ is the end that
sticks to hair, socks, shirts
5’ end is pointy/spiky
3’ end is soft/furry
Wielding the Power
• ‘Recall’ that ribosome assembly is the
result of methionine tRNA finding a
match on mRNA in presence of small
ribosome subunit
• Only methionine tRNA (it will ‘know itself’
once crowned by the synthetase that
hands out met) can team with small
ribosomal subunit & join with the
‘AUG’!
Ready…. Go!
• mRNA at the central bench
• ribosome assembles around it
• synthetases at bench corners (or
‘diffuse’ opp. direction vs. tRNA)
• tRNAs will ‘diffuse’ by following a path
through the room
• When any event first happens*, action
stops, molecules involved will
announce, explain Includes ‘didn’t work’
• Go until a protein happens
“Who” knows what’s
going on?
• What happens if a tRNA carries the
wrong amino acid?
• What happens if the mRNA contains a
copy error relative to DNA?
• What happens if a tRNA has a mutated
anticodon
Exit Condition
1.) Pair up (two in a group)
2.) Write your names at the top of the paper
3.) EXPLAIN the process of TRANSLATION
Include the following in your answer:
tRNA
mRNA
ribosome
AUG codon
RNA Polymerase
aminoacyl tRNA synthetase
termination factor
diffusion
Meet your new best friend
http://www.youtube.com/watch?v=-zb6r1MMTkc