Transcript Supplementary Figures - PowerPoint

Supplementary Figure Legends
Supplementary Figure 1 | Phylogenetic relationships of HA genes from H5N1
influenza viruses. Viruses used in this study are shown in color: human isolates in
red and avian isolates in blue. The viruses were classified into 4 clades according
to the published classification system (1).
Supplementary Figure 2 | Receptor specificity of H5N1 viruses. Direct binding
activity to sialylglycopolymers containing either α2,3-linked (blue) or α2,6-linked
(red) sialic acids was tested. a, Viruses isolated from avian species. b-d, Viruses
isolated from humans or viruses generated by reverse genetics based on the HA
sequences of human isolates in the Influenza Sequence Database. In all panels,
the results are represented as means ±standard deviations of triplicate
experiments.
Supplementary Figure 3 | Specificity of the receptor assay. Binding of (a) lectins
and (b) viruses to sialylglycopolymers, asialoglycopolymers and polymer backbone.
Sialylglycopolymers containing either α2,3-linked (blue) or α2,6-linked (red) sialic
acids and the polymer lacking oligosaccharides (black) were treated (open circle)
or mock-treated (closed circle) with Arthrobacter ureafaciens sialidase and then
incubated with MALI, MALII, SNA lectin, or viruses, as indicated. The data are
from a single representative experiment.
Supplementary Figure 4 | Effect of HA mutations on SAα2,6Gal recognition.
Mutations found in the HA of A/Vietnam/30408/05clone7 were introduced
individually or in combination into the reference VN1194 HA. Direct binding activity
to sialylglycopolymers containing either α2,3-linked (blue) or α2,6-linked (red) sialic
acids was tested. a, VN1194 HA and viruses with a single HA amino acid change.
b, Viruses with two amino acid changes. c, Viruses with three amino acid changes
and the A/Vietnam/30408/05clone7RG. Mutations introduced into the VN1194 HA
are shown in parentheses. In all panels, the results are represented as means
±standard deviations of triplicate experiments.
Supplementary Reference
1. World Health Organization Global Influenza Program Surveillance Network. Evolution
of H5N1 avian influenza viruses in Asia. Emerg. Infect. Dis. 11, 1515-1521 (2005).