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Overview JGI RAST Manatee Length 7130 10002 10002 Description The Glug motif Halomucin [Precursor] Halomucin Table designed by Jay McNair Gene 2500590430 10002 JGI 869 Rast 943 Manatee 844 0 2000 4000 6000 8000 10000 http://gcat.davidson.edu/GcatWiki/index.php/Gene_Len gth_Histograms 12000 GLUG Motif http://pfam.sanger.ac.uk/ GV GV GV PHB PHB GV GV PHB GV Electron Tomographic image of a single square cell of Haloquadratum walsbyi http://www.biomedcentral.com/14712164/7/169 A Signal sequence VGGL(x)35 repeat D/S-rich domain D-rich domain Halomucin (9195 amino acids) B 1 20000 1 40000 2 3 4 5 6 60000 7 hmu1 capCB C D bp 1031 800 600 400 M 1 2 3 4 5 6 7 8 9 M H. walsbyi encodes halomucin, an extremely large protein homologous to mammalian mucins. GC content (C) and gene organization (B) of a 60.000 bp long region of the genome of H. walsbyi. This region contains the large, high GC gene encoding halomucin (hmu1) and the two capsular protein biosynthesis genes capC and capB. The domain organization of halomucin (A) reveals domains typically found in mammalian mucins. The letters D, S, V, G, and L indicate domains enriched for the corresponding amino acids. Halomucin expression is shown by detection of its mRNA via RT-PCR (D). cDNA was prepared by reverse transcription using primer sets numbered 1 to 7 the position of which correspond to the striped and numbered boxes in the hmu1 gene (B). http://www.biomedcentral.com/1471-2164/7/169 tblastx Resuts 3 frameshifts mentioned in halomucin paper