The Use of Self-Patterned Phospholipid Films for Directed Enzyme Lithography
N.Y.-W. TANG and A. Badia, Department of Chemistry, University of Montreal,
C.P. 6128, succursale Centre-ville Montreal, Qc, H3T 1J4, [email protected]
Langmuir-Blodgett Monolayers of DPPC/DLPC
Generate phospholipid-based surface patterns from phase-separated monolayers
using the Langmuir-Blodgett technique
PLA2, a calcium-dependent and interfacially-active
enzyme, catalyzes the cleavage of the sn-2 acyl ester
linkage of glycerophospholipids, yielding a fatty acid and
Study the effect of lineactants on the pattern morphology
Demonstrate that a striped phospholipid bilayer prepared by the
Langmuir-Blodgett technique can be used for enzyme nanolithography
The naturally occuring L form of the lipid is hydrolyzed,
PLA2 binds but does not cleave the D form.
Structures of Phospholipids and Lineactant
DPPC, C16; Tm = 41°C
PLA2 Degradation of a Striped D--DPPC/L--DLPC Bilayer
Stripe Formation Mechanism
DLPC, C12; Tm = -1 °C
Langmuir Monolayers of Phospholipids
30 40 50 60 70 80 90 100 110
Area (Å /molecule)
P. Moraille and A. Badia, Langmuir 2002, 18, 4414-4419
PLA2 Degradation of a Striped L--DPPC/ DEPC Bilayer
Solid-Supported Phospholipid Films
Effect of Cholesterol Lineactant on the Domain
Morphology for a 50/50 DPPC/DLPC Mixture
at = 32 mN/m
Atomic Force Microscopy (AFM)
Characterization of the Surface Morphology
Conclusions and Future Work on Enzyme Nanolithography
Enzymes make interesting nanolithographic tools due to their ability to catalyze
chemical reactions with high efficiency and specificity under mild conditions and
The stereospecificity exhibited by certain enzymes can be exploited to spatially direct
their activity to certain regions of a patterned surface, as shown herein.
We have investigated the phospholipase A2 (PLA2 ) hydrolysis of stereochemically-differentiated
DPPC/DLPC and chemically-differentiated DPPC/DEPC bilayers.
PLA2 stereospecifically hydrolyzes the L--phospholipid enantiomer in bilayer templates.
PLA2 only hydrolyzes dialkylphosphatidylcholines in bilayer templates.
The regularity of the monolayer and bilayer stripe patterns will be improved.
The PLA2 enzyme performance will be demonstrated for different enantiomer templates .
Alkyl tail-functionalized phospholipids will be used to spatially direct the deposition of metal nanoparticles
for the generation of 2D optical gratings.