Transcript AP Bio Lab Changes

 Instructors
can continue to use
most of the “Dirty Dozen” as long
as an inquiry-based approach is
applied.
 Use of calculators
 Formula sheet
 Inquiry-based investigations
 Inquiry-based
investigations
 Emphasis on a variety of reporting
strategies
 Longer timeframe (still 25% of time
but each investigation builds on
initial understanding to produce
authentic investigation)
 Circulatory
System
 Aquatic Primary Productivity
 Investigation
1: Artificial Selection
 Investigation 3: Bioinformatics
(BLAST)
 Artificial
Selection
 Modified Animal Behavior
› Fruit Flies
› Choice chambers
› Food preferences
› Light and Dark
› Temperature
› Taxis behaviors
 Hardy-Weinberg
 Computer-based
modeling using
Excel® spreadsheet
 Alternative labs
› BioQUEST - http://bioquest.org/
› HHMI -
http://www.hhmi.org/biointeractive/ev
olution/pocket_mouse_evolution.html
 BLAST
– DNA sequence
comparisons
 Evolutionary Relationships /
Cladograms
 Bioinformatics database to
compare nucleic acid, protein
sequences to establish
evolutionary relationships
 Alternatives
 Focus
topic – The origin of human
malaria parasite (P. falciparum)
 Diffusion
/ Osmosis
 Added cell size diffusion activity
 Retains dialysis component
 Retains water potential
 Photosynthesis
 Replaces
DPIP experiment with
floating leaf disc assay
 Retains pigment chromotography
 Cellular
Respiration
 Microscale using smaller seeds
 Alteratives
› Animal Models with respiration
chambers
› Standard set up still okay
 Cell
Division: Mitosis and Meiosis
 Uses lectin-like substance to alter
mitosis
 Cell staining to evaluate
differences in mitotic activity
 Cell cycle control case studies –
HeLa cells, Philadelphia
chromosomes
 Bacterial
Transformation
 Same as always
 Lower opportunity for inquiry
 Suggestions????
 Restriction
Enzyme Lab
 Forensic introduction
 Could include enzymatic
digestion rather then predigested
samples
 Many kits available with forensic
spin
 Energy
Dynamics (replacement
for Aquatic Primary Productivity)
 Attempts to compare primary
producers to primary consumers
 Long timeframe, less than direct
comparison
 Any
online trophic level activity
 BioQUEST
 Transpiration
(w/ or wi/ sensors)
 Still potometer EEEKKKK
 Please go whole plant. It avoids
all the technical difficulties.
 Takes a little longer, but worth it!!
 Animal
Behavior changed to fruit
fly food preferences
 Inexpensive
 Easily adapted to inquiry-style
 Enzyme
Catalysis
 Change to turnip peroxidase from
catalase
 Great opportunity for inquiry
 Works easily – but guaiacal very
“aromatic”