Transcript Biochemistry 6. Proteins - Hatboro

Nucleic Acids
Information
storage
Nucleic Acids
• Function:
– genetic material
• stores information
– genes
– blueprint for building proteins
» DNA  RNA  proteins
DNA
• transfers information
– blueprint for new cells
– blueprint for next generation
proteins
DNA  RNA  protein: information
flow in a cell
DNA
1 Synthesis of
mRNA in the nucleus
mRNA
NUCLEUS
CYTOPLASM
mRNA
2 Movement of
mRNA into cytoplasm
via nuclear pore
Ribosome
3 Synthesis
of protein
Polypeptide
Amino
acids
Nucleic Acids
• Examples:
– RNA (ribonucleic acid)
• single helix
– DNA (deoxyribonucleic acid)
• double helix
• Structure:
– monomers = nucleotides
RNA
Nucleotides
• 3 parts
– nitrogen base (C-N ring)
– pentose sugar (5C)
• ribose in RNA
• deoxyribose in DNA
– phosphate (PO4) group
Are nucleic acids
charged molecules?
Nitrogen base
I’m the
A,T,C,G or U
part!
Types of nucleotides
• 2 types of nucleotides
– different nitrogen bases
– purines
• double ring N base
• adenine (A)
• guanine (G)
– pyrimidines
•
•
•
•
single ring N base
cytosine (C)
thymine (T)
uracil (U)
Purine = AG
Pure silver!
Nucleic polymer
• Backbone
– sugar to PO4 bond
– phosphodiester bond
• new base added to sugar of previous
base
• polymer grows in one direction
– N bases hang off the
sugar-phosphate backbone
Dangling bases?
Why is this important?
Pairing of nucleotides
• Nucleotides bond between
DNA strands
– H bonds
– purine :: pyrimidine
– A :: T
• 2 H bonds
– G :: C
• 3 H bonds
Matching bases?
Why is this important?
DNA molecule
• Double helix
– H bonds between bases join
the 2 strands
• A :: T
• C :: G
H bonds?
Why is this important?
Copying DNA
• Replication
– 2 strands of DNA helix are
complementary
• have one, can build other
• have one, can rebuild the whole
Matching halves?
Why is this
a good system?
When does a cell copy DNA?
• When in the life of a cell does DNA have to
be copied?
– cell reproduction
• mitosis
– gamete production
• meiosis
DNA replication
“It has not escaped our notice
that the specific pairing we
have postulated immediately
suggests a possible copying
mechanism for the genetic
material.”
-James Watson & Francis Crick
(1953)
1953 | 1962
Watson and Crick … and…
Maurice Wilkins… and…
1953 | 1962
Rosalind Franklin (1920-1958)
X-ray crystallography
X-ray
diffraction pattern
Photographic film
Diffracted X-rays
X-ray
source
X-ray
beam
Crystal
(a) X-ray diffraction pattern
Nucleic acid
Protein
(b) 3D computer model
Interesting note…
• Ratio of A-T::G-C
affects stability
of DNA molecule
– 2 H bonds vs. 3 H bonds
– biotech procedures
• more G-C =
need higher T° to
separate strands
– high T° organisms
• many G-C
– parasites
• many A-T (don’t know why)
Another interesting note…
• ATP
Adenosine triphosphate

modified nucleotide
 adenine (AMP) + Pi + Pi
+
+
Review Questions
1. What substance varies within a nucleotide
of DNA?
A. Deoxyribose
B. Nitrogenous Base
C. Phosphate Group
D. Ribose
E. Sugar
2. All of the following bonds are correct
EXCEPT:
I. A Ξ T
II. C Ξ G
III. A Ξ U
A.
B.
C.
D.
E.
I only
II only
III only
I and II only
I and III only
3. A sample of double-stranded DNA has equal
numbers of
A. Adenine nucleotides and guanine nucleotides.
B. Adenine nucleotides and cytosine nucleotides.
C. Cytosine nucleotides and Thymine
nucleotides.
D. Purines and pyrimidines.
E. Thymine and Uracil Molecules.
Macromolecule
Review
Carbohydrates
• Structure / monomer
– monosaccharide
• Function
– energy
– raw materials
– energy storage
– structural compounds
glycosidic bond
• Examples
– glucose, starch, cellulose, glycogen
Lipids
• Structure / building block
– glycerol, fatty acid, cholesterol, H-C chains
• Function
– energy storage
– membranes
– hormones
• Examples
– fat, phospholipids, steroids
ester bond (in a fat)
Proteins
• Structure / monomer
– amino acids
– levels of structure
• Function
– enzymes  defense
– transport  structure
– signals  receptors
peptide bond
• Examples
– digestive enzymes, membrane channels, insulin
hormone, actin
Nucleic acids
• Structure / monomer
– nucleotide
• Function
– information storage
& transfer
• Examples
– DNA, RNA
phosphodiester bond