Transcript In Vivo Xenograft Services PowerPoint Presentation

Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Xenografting
 Xenografting is the transplantation of
tissue from one species into another.
 Xenografting has been established as
benchmark studies in pre-clinical
cancer research.
 Typically, immunodeficient mice serve
as hosts for a wide variety of human
tumors, effectively serving as models
for human subjects.
 Xenografting a complete and accurate
study of tumor growth and the activity of
drug administration.
Xenografting tumor cells into
mice has advanced pre-clinical
cancer research significantly
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Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Advantages of Xenografting
 Develop new therapeutic agents
Xenotransplantation is
the transplantation of
living cells, tissues or
organs from one
species to another.
quickly, efficiently and cost effectively
 Evaluate the efficacy and toxicity of
potential therapeutic agents
 Evaluate target compound activity
using in vivo system (human cells)
 Predict cytotoxicity of cancer drugs
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Xenograft Study Pipelines
 Pipeline Xenograft Assay I
Our staff designs the study based on your project
requirements and objectives. Leveraging years of
knowledge and experience, Altogen Labs guarantees
an optimized experimental approach with respect to
mice selection, cell lines, inoculation, and dosage.
 Pipeline Xenograft Assay II
 Testing a Compound
The client provides a compound and cell line to be
researched. Our staff will establish a xenograft
animal model and test compound activity and how it
affects tumor growth.
 Pipeline Xenograft Assay III
Representative data of
a xenograft compound
test study
Nature (2010) Sep 30;467(7315):596-9
 Testing a Cell Line
The client provides a cell line and our staff tests its
ability to establish tumor growth in standard
immunodeficient mice.
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Compound Testing Objectives
 How does the test compound affect tumor growth?
 Does the test compound inhibit tumor invasion or metastasis?
 Localization of test compound in the tumor?
 Define drug characteristics:
Bioavailability
 Toxicity
 Efficacy
 Potency
 Is the cell line tumorigenic?
 Changes in gene expression upon treatment or gene
manipulation
 How does gene manipulation in a cell line tumor growth,
invasiveness, and metastasis
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Xenograft studies can
rigorously characterize
candidate drug efficacy
and pharmacokinetic
properties
http://news.mit.edu
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Basic Xenograft Study
Xenograft studies can be designed to:
 Identify lead compounds
 Optimize dose schedules
 Identify combination strategies
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
 Xenograft Service Options
 Use in-house or customer-provided cell lines
 150 in-house cell lines available
 Begin testing a compound immediately after injecting mice
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with tumor cells (unstaged) or once the tumors reach a certain
size (staged)
Assign mice to groups randomly or stratify them by body
weight, tumor size, disease factors, or any combination of
parameters
Administer test compounds by any of the following methods:
topically, IP, IV, SC, PO, high pressure tail vein, osmotic
minipumps (SC or IP), SC drug pellets, or in the food or water
Snap freeze harvested tumors or fix them for histology or
other specialized assays
Analyze blood chemistry at various times throughout the
study
MCF-7 cells LNCap cells
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Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
pathpedia.com
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
 Growth Rates for Different Cell Lines
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
 Sample Cell Lines Available
Cell Line
Tissue
Disease
Caki-2
Kidney
Clear Cell Carcinoma
PANC-1
Pancreas
Epithiloid Carcinoma
SK-MEL-2
Skin
Malignant Melanoma
SK-OV-3
Ovary
Adenocarcinoma
U-87 MG
Brain
Glioblastoma/Astrocytoma
A431
Epidermis
Epidermoid Carcinoma
SW-480
Colon
Adenocarcinoma
HeLa
Cervix
Adenocarcinoma
Altogen Labs has ~150 cell lines available for xenograft animal studies
Complete listing available at altogenlabs.com/xenograft-models
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Drug Administration Variables
 Route of dose:
Intratumoral
 Intramuscular
 Oral
 Intravenous
 Intratracheal
 Subcutaneous
 Intraperitoneal
 Frequency of dose
 Duration of dose
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Several routes of drug
administration can be
explored in a
Xenograft model
www.patient-derived-xenograft-services.com
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Data Endpoints
 Estimated tumor volume (L x W2
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/2)
Mean/median time to specified
tumor volume
Survival time to specified tumor
volume
Tumor doubling time
Tumor growth inhibition (TGI)
Tumor growth delay (TGD)
Increased life span (ILS)
Tumor cell kill rate
A549 (Lung Carcinoma) Xenograft
Study
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Expanded Xenograft Services
 Clinical observations daily
 Haematology analysis (blood cell count)
 Clinical chemistry profile (of urine and
plasma)
 Assessment of metastasis
 Characterization of the tumor (necrosis, etc)
 Tumoral tissue will be banked and
preserved for further analysis
 Cryo-preservation (for cell culture)
 Formalin fixed tumors and tissues (for
histopathological and immunostaining
analysis)
 RNAlater fixed tumors and tissues (for RNA
isolation and gene expression analysis)
Our services include expanded
tumor cell characterization and
preservation
cryostuff2.com
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]
Provider of Global Contract Research Services
Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
 Altogen Labs’ team of scientists carries
extensive experience in Xenograft model
research
 Altogen Labs is compliant as a Good
Laboratory Practices (GLP) environment,
which is required by the FDA for for preclinical research.
 Our Altogen Labs’ Xenograft services are
extensive, rigorous, and flexible.
Altogen Labs can partner
with you for any Xenograft
research project.
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Contact us to discuss Xenograft model details, timeline estimates, and
price quotes!
Altogen Labs  11200 Manchaca Road #203  Austin  TX  78748  USA
Telephone  512 433 6177  email  [email protected]