Transcript OPLC principles

PLANT DRUG ANALYSIS BY
OPTIMUM
PERFORMANCE
LAMINAR
CHROMATOGRAPHY
Jacques POTHIER,
Laboratory of Pharmacognosy Tours,
France
North Carolina University july 14 th 2003
OPLC 50
historique
• École hongroise Erno TYIYAK Emil
MINCOVICS
• CCM rapide
• En fait HPLC sur colonne plate
OPLC principles:
the « flat column » concept
External pressure 50 bars
Solvent in
Micro groove
Way out
Teflon sheet
Sorbent bed (silica)
Metal cassette
From OPLC 1 to OPLC 3
• In the begining OPLC was known as
Overpressured layered chromatography
– 1 chrompress 25
– 2 personal oplc
– 3 to day OPLC is optimal performance
laminar chromatography Forget the
previous methods
– The third generation is dependable, realiable
and without surprises.
What is OPLC ?
• Planar and liquid chromatographic techniques
• Hybrid technique between TLC and HPLC
• Enabling high performance separation of single
sample or of several samples in parallel
– Laminar mobile phase
– Gradient capabilities
– Under pressure excluding vapor phase
• Planar columns
– Disposable, convenient providing laminar flow
– Industry selection of phases and particle sizes (5µm,
11µm)
OPLC 50
From OPLC 1 to OPLC 3
• In the begining OPLC was known as
Overpressured layered chromatography
– 1 chrompress 25
– 2 personal oplc
– 3 to day OPLC is optimal performance
laminar chromatography Enhanced over
previous methods.
– The current generation is the latest OPLC.
OPLC principles:
the « flat column » concept
External pressure 50 bars
Solvent in
Micro groove
Way out
Teflon sheet
Sorbent bed (silica)
Metal cassette
OPLC/HPLC
• In OPLC the column is always new. With
HPLC...?
• En oplc toujours possible de vérifier la
migration
• With OPLC, all eluents can be used, acidic
and basic.
• Peut servir pour transposition ccm hplc