Transcript Research Core Facilities - Vanderbilt University Medical Center

Vanderbilt Research Shared Resources and Core Facilities
Susan Meyn
Director – Research Resources
VUMC Office of Research
Agenda
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Role of the Office of Research
VUMC Strategic Plan and Core Facilities
VUMC Core Facilities – Overview
How VUMC Core Facilities can support your
Research Efforts
• VUMC Core Facilities – Highlights
• Resources
ROLE OF THE OFFICE OF RESEARCH
Office of Research:
General resources and support for research
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Point of contact for the research community both on campus
and outside the institution
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Funds and coordinates development of specialized
infrastructure (equipment, facilities) to support multiple
research efforts
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Provides grant-writing resources including grant-ready text
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Coordinates limited submission opportunities and institutional
support for selected grant programs
contact email: [email protected]
website: http://vanderbilt.edu/oor/
Office of Research
Associate Vice
Chancellor for
Research
Office of
Research
Shared
Resources
Oversight
Committee
Home Center or
Department
Core Advisory
Committee
Core Facility
Office of Research:
Core Facilities and Shared Resources
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Supports scientific oversight and development of new shared
resources, continued development of existing cores
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Works with research centers and programs to keep core
facilities on the cutting edge by providing new equipment,
recruiting technical staff.
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Coordinates with VICTR funding program to provide research
support.
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Oversees and administers the Scholarship program in
conjunction with NIH-funded centers to provide assistance with
core service fees.
contact email: [email protected]
website: http://vanderbilt.edu/oor/
VUMC STRATEGIC PLAN
AND CORE FACILITIES
Vanderbilt’s Research Enterprise Strategic Plan and
Core Facilities
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Personalized Health & Healthcare
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Therapeutic Discovery & Translation
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Public Health & Healthcare
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Foundations of Basic Science
Leverages network of shared core facilities in Centers developed through
expansion of space, faculty, and $100M investment.
2005–2010
VANDERBILT UNIVERSITY MEDICAL CENTER
RESEARCH ENTERPRISE
STRATEGIC PLAN
http://www.vanderbilt.edu/oor/about/strategic-plan12-2005
Vanderbilt’s Research Enterprise Strategic Plan and
Core Facilities
•
Personalized Health & Healthcare
•
Therapeutic Discovery & Translation
•
Public Health & Healthcare
•
Foundations of Basic Science
Leverages network of shared core facilities in Centers developed through
expansion of space, faculty, and $100m liquidation of quasi-endowment (20002005).
2005–2010
VANDERBILT UNIVERSITY MEDICAL CENTER
RESEARCH ENTERPRISE
STRATEGIC PLAN
http://www.vanderbilt.edu/oor/about/strategic-plan12-2005
Research cores/shared resources
Discovery
Integrative
science
Individual
biomarkers
Disease pathways
Drug targets
Model systems
Genomics
Proteomics
Metabolomics
Imaging
Personalized Medicine
• Disease susceptibility
• Early detection & prevention
• Variable drug actions
Implementation
Translation
VUMC CORE FACILITIES –
OVERVIEW
Overview –
Vanderbilt Institutional Cores
Medical Research Building III (MRBIII)
Mass Spectrometry Resource Center
Neurochemistry Core Laboratory
Biomolecular Crystallography Facility
Biophysical Instrumentation Core Facility
Cell Imaging Shared Resource
Medical Center North (MCN)
CRC–Clinical Research Center
Division of Animal Care
Comparative Pathology
Biostatistics Collaboration Center
Flow Cytometry and Immunology Cores
Medical Research Building IV (MRBIV)
Transgenic Mouse/ESC Shared Resource
VICB Chemical Synthesis Core
Cell Imaging Shared Resource
Medical Center North (MCN)
Sleep Research Core
Murine Neurobehavioral Laboratory
Rat Neurobehavioral Laboratory
Vanderbilt University Institute of Imaging Science
Relocating soon (VANTAGE):
Genome Sciences Resource & BioVU
DNA Resources
Light Hall
Cell Imaging Shared Resource
Molecular Cell Biology Resource Cores
Mouse Metabolic Phenotyping Center
ZCORE - Zebrafish Aquatic Facility
Robinson Research Building
VICB High-Throughput Screening Facility
Preston Research Building
Cancer Biostatistics Center
Epidemiology Biospecimen Core
Hormone Assay and Analytical Services Core
VICB Protein and Antibody Resource
The Vanderbilt Clinic (TVC)
Human Tissue Acquisition and Pathology
Investigational Drug Services
Not shown:
Computational Genomics
Genetic Studies Ascertainment
Survey Research Shared Resource
ACCRE - Advanced Computing Center
Biomolecular NMR Facility
Research core or shared resource:
• Is a research laboratory accessible to
multiple investigators across disciplines.
• Provides specialized or technologically
advanced laboratory services, equipment
and expertise.
• Often based in an NIH funded center, e.g.
the Cancer Center.
• Dedicated personnel include scientific
manager/director and technical staff.
• Supported by chargebacks to investigator
grants.
Typical Core Structure
Research Center
or Department
Faculty – Core
Scientific Director
Core Manager
or Operations
Director
Core research
technicians
Senior Technical
Specialists
HOW VUMC CORE FACILITIES CAN
SUPPORT YOUR RESEARCH EFFORTS
VUMC Core Facilities support your Research Efforts
More than 30 institutional cores and shared resources –
Whatever your research interest, there is a core facility that can
offer high end equipment access, specialized technology or
advanced scientific expertise.
• $1 of every $10 funded by NIH goes to cores at Vanderbilt.
• Vanderbilt is nationally recognized for the quality and
efficiency of its core facilities.
• Vanderbilt is a leader in core technology development.
Scientists at all levels come to Vanderbilt because of our cores –
make these valuable resources part of your research plan.
VUMC Core Facilities support your Research Efforts –
Grant Funding
Grant applications – collaborate with a core:
• Plan your project and define the scope of services
• Request core service fees in your budget
• Do not request personnel costs – i.e. no % effort
Reviewers are very positive about using core facilities to
accomplish research aims.
VUMC Core Facilities support your Research Efforts –
Technical Support
Experimental design, planning, troubleshooting:
Not sure how to start?
• Take a specific core training session
• Attend a core seminar
• Stop by the core to talk with core technical staff
• Not finding the technology that you need? Contact the Office of
Research, or search the core listings.
• Core facilities help you achieve your research goals.
VUMC Core Facilities support your Research Efforts Support to pay core service charges
Unfortunately, use of research core facilities is not free! BUT
there are sources of funding that can help you pay the bill:
• Join a research center - for example:
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Cancer Center
Diabetes Center
Vision Research Center
Digestive Disease Research Center
• Members of research centers are eligible for core
scholarships – credit vouchers that can be used to pay core
service charges in supported core facilities.
• Apply for pilot project funding or micro-grant vouchers
from VICTR. Investigators may receive up to $2000 every
six months to pay for core services.
VUMC CORE FACILITIES –
HIGHLIGHTS
Center for Small Animal Imaging – MRI, PET, SPECT, CT, Ultrasound
Center for Human Studies – MRI and full range of ancillary equipment
Cell Imaging Shared Resource – Confocal laser scanning microscopes,
TEM, image processing workstations
Imaging
Cell Imaging Shared Resource
Cell Imaging Shared Resource
Mass Spectrometry Resource Center – Mass Spec, Proteomics
Tissue Imaging
Center for Structural Biology – NMR, X-ray crystallography,
Biophysical instrumentation
Institute for Chemical Biology – High Throughput Screening and
Chemical Synthesis
Proteomics
Mass Spectrometry Resource Center
in rat, 2 hr post-dose
Optical image
Olanzapine
m/z 313256
N-desmethylm/z 299256
2-hydroxymethylm/z 329272
Khatib-Shahidi, S. et al., Anal Chem, 2006, 78, 6448
http://pubs.acs.org/doi/pdf/10.1021/ac060788p
Cell-based Assays, Animal Models
Flow Cytometry & Immunology cores – cell surface and organelle
markers, cytokine assays
Transgenic Mouse – Embryonic Stem Cell Shared Resource
Mouse and Rat Neurobehavior cores
Flow Cytometry Core
A
CSF
7%
4%
Blood
100%
4%
4%
Memory
Markers
88%
30%
14%
CD45RO
CD62L
CD127
70%
50%
57%
CD45RO
8%
45%
CD62L
39%
Homing
Markers
68%
52%
CCR5
VLA4
37%
7%
33%
CCR5
VLA4
16%
Activation
Marker
28%
12%
CD38
37%
42%
CD127
Tetramer+ HIV-specific CD8+T cells
100%
B
CD38
100%
36%
100%
Exhaustion
Markers
24%
74%
CD57
PD-1
23%
39%
CD57
PD-1
Figure 2: Distribution of memory, homing, activation and exhaustion markers. Cells from CSF (A) and blood (B) of a HIV+ patient were
directly stained with a 14-colour antibody panel. Flow cytometric plots of total CD8+ T cells (in blue) are shown with HIV-specific CD8+ tetramer+
(B7-TL10) T cells on the Y axis (in green) and memory maturation markers (CD45RO, CD62L and CD127), homing markers (CCR5, VLA-4),
activation markers (CD38) or markers of T cell exhaustion (PD-1 and CD57) on the X-axis. Percentages on bottom and top of each plot represent
frequencies of markers on total CD8+ T cells and HIV-specific T cells respectively.
Enhanced PD-1 expression by T cells in cerebrospinal fluid does not reflect functional exhaustion during chronic human immunodeficiency virus type 1 infection.
Sadagopal S, Lorey SL, Barnett L, Sutherland D, Basham R, Erdem H, Kalams SA, Haas DW.
J Virol. 2010 Jan;84(1):131-40. Epub .
DNA Resources – extract, bank and genotype DNA
BioVU - DNA and de-identified clinical databank
Genome Sciences Resource – microarrays, next-gen sequencing
(HiSeq, MiSeq)
Genomics
RESOURCES
Vanderbilt University Postdoctoral Research
and Shared Resources Symposium
Wednesday, May 2nd, 2012
Light Hall North Lobby and Langford Lobby
 Invited Presentations: 10:30 am - 1:00 pm, LH 208
* Lunch tickets provided for first 50 attendees prior to 10:30 am *
10:30 am
Dr. David Piston
Louis B. McGavock Chair, Professor of Molecular Physiology
& Biophysics, Physics, & Chemical & Biomolecular
Engineering
11:00 am
Dr. Kevin Schey
11:15 am
Dr. Daniel Foster
Director, VVRC Proteomics Core Facility
Vanderbilt Center for Neuroscience Drug Discovery
“Elucidation of M5-muscarinic receptor
neurophysiology in midbrain dopamine neurons using
the novel allosteric modulator VU0238429”
11:30 am
Dr. Christopher Coldren
Operations Director, Genomic Sciences Resource
11:45 am
Dr. Xiaojing Wang
Department of Biomedical Informatics
“customProDB: an R package to generate customized
protein database from RNA-Seq data for proteomics search”
12:00 pm
15 minute break
Lunch provided for first 50 attendees in LH 208
12:15 pm
Dr. Sam Wells
Director, Cell Imaging Shared Resource
12:30 pm
Dr. Thomas Kehl-Fie
Department of Pathology, Microbiology & Immunology
“Manganese sequestration by the host is an essential
component of innate immunity”
12:45 pm
15 minute break
 Poster Sessions: 1:00 pm - 3:00 pm, LH Lobby & Langford
Lobby * Light refreshments will be provided in Langford Lobby*
Session A, LH Lobby
1:00 - 2:00 pm
Session B, Langford Lobby
2:00 - 3:00 pm
Animal/Clinical Models
Biochemistry
Biological Sciences
Cancer Biology
Cell Biology
Microbiology/Immunology
Molecular Physiology & Biophysics
Neuroscience
Pharmacology
Keynote Address: 3:30 pm - 4:30 pm, LH 208
Dr. Gregory S. Kopf
Associate Vice Chancellor of Research Administration,
University of Kansas Medical Center
Former Assistant Vice President at Wyeth Pharmaceuticals
“Weathering the “Perfect Storm”: Charting the Course for a Career
in the Life Sciences ”
 Awards Ceremony: 4:30 pm, LH 208
Dr. Richard Peek, 2011 Mentor of the Year
“Perspectives & Philosophies on Mentoring”
Presentation of “Mentor of the Year” Award
Presentation of “Postdoc of the Year” Award
Poster awards: “Best in Category”
“Best Use of Shared Resources”
Many thanks to our generous Vanderbilt sponsors!
BRET Office of Postdoctoral Affairs & Roger Chalkley
Office of Research & Shared Resources
Vanderbilt Medical Alumni Association
Postdoctoral T32 Training Grant Directors
http://vanderbilt.edu/oor/
Core Listings
http://vanderbilt.edu/oor/
Grant ready text, etc
https://starbrite.vanderbilt.edu/
C.O.R.E.S.
Core Ordering and Reporting Enterprise System
www.cfuis.mc.vanderbilt.edu
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Web-interface
Place orders directly with core facilities
Reserve time on core equipment
Review and act on charges made against your grants or VICTR
awards
Questions?