Transcript Nanophysiometer Michael Ackermann1 - Research

Instrumented Nanophysiometer for High Throughput Drug Delivery
D. Michael Ackermann1, Jonathan Payne1, Hilary Samples1, James Wells1
Advisors: Franz Baudenbacher, Ph.D.2; Paul King, Ph.D.1
1
Objective
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN
2 Department of Living State Physics, Vanderbilt University, Nashville, TN
Device
Software Controller
• Design a nano-liter sized
nanophysiometer suitable for cell culturing
• Implement on-chip peristaltic pumps for
low volume flow through perfusion
• Incorporate thin film microelectrodes in
the inflow and outflow channels for
monitoring physiological parameters
• Develop a LabView user interface to
manage valve sequencing
Soft Lithography
Results
0.51 mm
d
Volume Displaced   0.2562 d
[1] S.R. Quake and A. Scherer, "From Micro to Nano Fabrication with Soft Materials", Science 290: 1536-40 (2000).
[2] M.A. Unger, H.-P. Chou, T. Thorsen, A. Scherer, and S.R. Quake, "Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography", Science 288: 113-116 (2000).
Peristaltic Pumping
3-Valve Pumping Sequence
Stage
1
Valve
2
Flow Direction
Future Work
3
1
2
4-Valve Pumping Sequence
3
Stage
4
Valve
1
Closed
Valve
Open
1
Valve
2
pH Monitoring
• Use integrated thin film microelectrodes to monitor physiological parameters
– pH, glucose, etc.
• Electrodes coated with a substrate specific oxidase
– Catalyze reaction producing H2O2
– H2O2 then detected
• Pump Sequencing (# of valves)
• Cells
• Electrodes
Acknowledgments
3
4
2
Flow Direction
3
3