Transcript Li Hao - USD Biology

Chronic Ethanol Potentiates the Effect of
Neuropeptide S
in the Basolateral Amygdala and Shows
Increased Anxiolytic
and Anti-Depressive Effects
Johan Enquist, Madeline Ferwerda, Anuradha Madhavan, Derek
Hok and Jennifer L Whistler*
Background
• Stress mediator
-- activate arousal and stress-responsive mechanisms
-- associated with orexin system and CRF system (Ox1
CRF1)
-- suppress anxiety-like behaviors in response to stress
• Addiction-related
-- effect on the reward system from drug abuse
Orexin 1 anta
NPSR anta
CRF 1 anta
Main Paper
Materials and Methods
Animals: male, 8 weeks old, mice.
Dose of NPS :3 nmol per animal
EtOH Consumption: 20% EtOH contingent access for 4 weeks.
Anxiety-like tests: Light/dark transition box, Elevated plus maze,
Open field test.
Depressive-like tests: Forced swim test, Tail suspension test.
Conditioned Place Preference(CPP)
Light/dark transition box
Forced swim test
Elevated plus maze
Tail suspension test
Open field test
Conditioned Place Preference
Elevated plus maze
Light/dark transition box
Open field test
Forced swim test
Tail suspension test
Animal had 1 hrs
between the infusion
and access to EtOH or
Water
EtOH Consumption
Animal had 4 hrs
between the infusion
and access to EtOH or
Water
Conditioned Place Preference
Whole cell patch-clamp on GABA neurons at BLA
Light/dark transition box
Injection of NPS into BLA
Take home message
NPS makes you happy.
Thank you!