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Integrated Enzyme-Logic systems for Monitoring
and Treating Autonomously Injured Soldiers
Joseph Wang (UCSD) and Evgeny Katz (Clarkson University)
Project Objectives:
To develop next-generation ‘sense
and treat’ autonomous devices for
enhancing the survival rate among A)
injured soldiers in the battlefield.
B)
Technical Approach:
Our
approach
involves
the
design,
optimization, and evaluation of novel
biochemical
cascades
composed
of
concatenated enzyme-based logic gates
capable of performing Boolean logic
operations on biomarker inputs.
This system will be applied to the
autonomous processing of physiological
information and interfaced with electronic
transducers & signal-responsive drug-delivery
materials / actuators.
By using multiple key injury biomarkers as
inputs for the enzyme gates, the biochemical
logic system provides high-fidelity diagnostics
when compared with single biomarker sensors.
400 μm
400 μm
Accomplishments/Impact/Transitions:
Extend enzyme logic gate functionality towards relevant injuries in
undiluted human serum samples with high fidelity
Developed enzymatic filters to enhance separation in readouts of
different input combinations.
First demonstration of the applicability of enzyme gates in a porcine model
of blunt liver injury
First demonstration of minimally-invasive microneedle sensor arrays for
monitoring fluctuations in key metabolites within the transdermal fluid
Developed wearable textile-based electronic transducers, μ-electronic
backbone to control logic gates
Developed signal-responsive materials and interfaces actuated by
enzyme-based injury related gates for targeted drug delivery and sustained
drug release formulation
Impact – Development of autonomous body-worn devices
/systems for minimally-invasive biomarker sampling
Transitioning towards ‘Pharmacy-on-Demand’ paradigm for
the controlled release of a therapeutic intervention in response
to signals generated by enzyme logic sensor