Transcript Baker final poster 2011_v4

Magnetically-Guided Nanoparticle Drug Delivery
Seth Baker, RET Fellow 2011
Percy Julian Middle School
RET Mentor: Prof. Andreas A. Linninger
Chicago Science Teacher Research (CSTR) Program – NSF-RET 2011
Introduction
Magnetite Nanoparticles
Superparamagnetic Properties
Motivation
• Direct application for improved medical treatments of neurological disorders
Biocompatible
cerebral artery
- Alzheimer’s, Parkinson’s, autism, cerebrovascular disease, abnormal vascular
structures (tumors), and stroke conditions.
• Improved pharmacokinetics and pharmacodynamics
- Limiting therapeutics to targeted sites reduces systemic distribution/toxicity
Superparamagnetic
relaxation
- Targeted delivery can lower dosage and reduce cytotoxicity
Spin glass
arrangement
Nanoscale
Functionalization
Objective
Many therapeutic drugs for treatment of neurological conditions can cause
systemic toxicity due to limited targeting of effected tissue. Magneticallyguided drug delivery offers treatment options that can reach site specific
areas of the brain. Testing is needed to determine a standard protocol for
infusing and guiding nanoparticles. Use of agarose brain phantoms can
eliminate preliminary animal testing.
Iron ions metabolize and
are biodegradable in vivo
Dipole alignment in the
presence of a magnet
Nanoparticles can be
coated with various agents
Experimental Design
Convection Enhanced Delivery
Step Catheters
Capillary Experiments
0.26 mm diameter
step catheter tip
Capillary experiment set 35 and 173 pound pull
173 pound pull force
up with 1.0 ml syringe
force magnets affect on magnet under capillary
and 30 nm magnetite
infused agarose gel
capillary experiment
Rat Brain Tests

New Era Pump System syringe pump

0.6% Agarose gel

Polyethylene tubing (various gauges)

Prussian Blue Stain

Polymer step catheters (various gauges)

Plastic cell blocks

1.0 ml medical syringes

Surfactants

Magnetic nanoparticles (various diameters)

Glass slides for slicing gel

Sodium Hydroxide

Canon EOS Rebel Xti

Magnets of various pull force

Rat brain tissue
Coronal slices of rat brain after
placed in Prussian blue dye to
determine untreated brain
susceptibility to staining.
Testing Magnetic Susceptibility
Results
Magnetic force was below the
injection site and syringe
needles were place ¼ inch
above magnet in each trial. Red
line indicates syringe placement.
30 nanometer Magnetite
particles above a 173 pound
pull force magnet at 0 minutes
30 nanometer Magnetite
particles above a 173 pound
pull force magnet at 4 minutes
Conclusion
• Magnetic nanoparticles indicate some
attraction toward a magnet during capillary
experiments in agarose gel brain phantoms.
• Step design for polymer catheters can
reduce reflux during convection enhanced
delivery of nanoparticles.
• Larger diameter nanoparticles tend to
agglomerate more rapidly than smaller
diameter particles.
30 nanometer Magnetite
particles above a 173 pound
pull force magnet at 8 minutes
Coronal slices of rat brain
showing distribution
profile of Prussia blue
dye.
30 nm magnetite particles
delivered on rat brain tissue
to determine susceptibility
to nanoparticles.
Control for capillary infusion
There is a general attraction of
magnetic nanoparticles through
the agarose toward the
magnets.
35 lb pull force magnet trial
173 lb pull force magnet trial
Acknowledgements
Future Studies
Improved infusion of magnetic nanoparticles
Studying various techniques to reduce the
agglomeration of magnetic nanoparticles through the
use of various surfactants as well as various catheter
design, tube diameters, and nanoparticle
concentrations.
Rat brain infusion
Improve methods of introducing magnetic nanoparticles into fresh brain tissue.
NSF CBET EEC-0743068 Grant, Chicago
Science Teacher Research (CSTR) Program
Director, A. Linninger

Members of LPPD, Andreas Linninger, Eric
Lueshen, Sukhi Basati, Indu Venugopal, Joe
Kanikunnel ,Bhargav Desai

RET Fellows at UIC
