Transcript Introduction Membrane Permeation System Experimental

Introduction
Membrane Permeation System
Experimental Section
Presented By: Rich Dominiak
Laura Kuczynski
John Roszko
Introduction
 Mass transfer through various membranes
is receiving increased attention
 Drug delivery through polymer membranes
and human or animal skin has become a
challenging research area
 In vitro setups are used to make
permeation measurements for membranemoderated controlled release of drugs
 The effect of the diffusion boundary layer
on permeation has not been studied
Introduction
 Permeation rate data can be easily distorted by
the diffusion boundary layers on the membrane
 The rate of drug permeation should be
measured using a calibrated permeation cell
 In this study
 The hydrodynamic characteristics of the an in-vitro
membrane permeation cell developed by Chien and
Valia were investigated
 Determine if it can be calibrated as a standardized invitro system
Membrane Permeation System
 Membrane surface area
– 0.64 cm2
 Star head magnetic
stirrer
 Donor and receptor
reservoirs
 3.5 mL capacity
 Completley enclosed
 Jacketed for temperature
control
Experimental Section
 Objective: To obtain the aqueous
solubilities of benzoic acid in various PEG
400 solutions
 Saturated solutions of benzoic acid in water
with 0-40% PEG 400 were prepared by
placing an excess of benzoic acid in test
tubes with the PEG solutions
 They were placed in a shaking water bath
for 24 hours
Experimental Section
 Filtered through 0.45 um Millipore
filter
 Diluted with solution medium that
was used in the test tubes
 Concentrations were analyzed using a
UV/vis spectrophotometer
 Viscosity was measured using and
Ostwald capillary viscometer
Experimental Section
 Diffusion coefficients were calculated
from literature values based on the
diffusivity in pure water at 25 C
 Wilke equation – 1.082 x 10-5 cm2/s
 Thin benzoic acid disks were made by
pouring fused benzoic acid into a
metal mold positioned on a pill tile
 Mounted to permeation cell
Experimental Section
 3.5 mL of each solution (previously
made) were pipetted into the
receptor compartment while the
donor compartment remained empty
 At time intervals, 20 uL were
withdrawn and diluted to 10 mL
samples
 These were analyzed with the UV
spectrophotometer