Transcript Blood tubes - Landis Foitik, RVT, BASVT

Blood tubes
Blood tubes
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Using the appropriate phlebotomy supplies
is imperative for accurate test results.
Each vacutainer tube is color-coded to
facilitate proper specimen collection.
Please be sure to use the proper tube for
the desired test.
Anticoagulants
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Hematology testing primarily uses whole blood
samples (Blood colection1%BW)
Hemostatic testing uses both whole blood and
blood plasma
An anticoagulant is a chemical that , when
added to the whole blood sample, prevents or
delays clotting (coagulation) of the sample
Regardless of the anticoagulant chosen, the
sample and anticoagulant must be well mixed
(by gentle inversion0 before use
Samples that are not tested within 1 hour of
collection should be refrigerated .
Lavender top
Potassium EDTA (K2
or K3 EDTA): For tests
requiring EDTA
plasma, separate
plasma appropriately.
Use for CBC and
blood cultures
Ethylenediamine tetraacetic acid (EDTA)
Green tops
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The interior of the tube wall is coated with
sodium heparin, lithium heparin, or
ammonium heparin
The anticoagulant heparin activates
antithrombins, thus blocking the
coagulation cascade and producing a
whole blood / plasma sample instead of
clotted blood plus serum
Green top
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Sodium Heparin:
Preferred heparin tube
for send out testing.
It is glass and Does Not
contain inert gel
For plasma
determinations in avian
and reptiles
Lt. Green top
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Lithium Heparin:
Contains an inert
gel for separating
plasma, which
acts as a barrier
between cells and
plasma after
centrifugation.
Red top
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Contains no anticoagulant. Used
in Blood Drug Screens and other
testing where the gel additive
could interfere with testing.
Red No Gel tubes are available in
"No Additive" clot tubes as well as
"Clot Activator" tubes for serum
collection.
Please take care to inspect the
tube label to identify the additive
type.
Use for serum determinations in
chemistry, serology and
Immunohematology (blood
banking).
TESTS -SEROLOGY
Red-Gray (tiger)
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Contains clot activator and
inert gel for separating serum,
which acts as a barrier
between cells and serum after
centrifugation.
During centrifugation the
barrier gel moves upward to
the serum - clot interface,
where it forms a stable barrier
separating the serum from
fibrin and cells.
Important
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For tests requiring serum, a blood sample is
drawn into a tube containing no anticoagulant.
The blood must be allowed to clot for
approximately 30 minutes before centrifugation.
Clotted blood should then be centrifuged for 1015 minutes.
The serum can then be separated from the cells
and transported in an aliquot tube, if necessary.
The use of a transfer pipette is the required
method, please DO NOT tilt the original tube
when separating the serum into an aliquot tube.
The presence of red blood cells may give
erroneous results
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It is the responsibility of all personnel who
collect laboratory specimens to properly
label the specimens to assure accurate
patient/sample identification.