Transcript Document

• Bioadhesion can be defined as the ability of a drug carrier
system (synthetic or biological) to adhere to a biological
substrate for an extended period of time.
•The biological surface can be epithelial tissue (skin) or the
mucous coat on the surface of a tissue.
•If the adhesive attachment is to a mucous coat, the
phenomenon is referred to as mucoadhesion.
Importance of Bioadhesion in Drug delivery
Bioadhesion technique used to optimize either local or
systemic drug delivery for various routes of administration
by:
a. Extension of Contact Time:
prolong contact time of a drug delivery system to biological
tissue can improve drug therapy.
b. Localization of Drug Delivery System:
Some drugs are preferentially absorbed in a specified region
"window for absorption". e.g., iron, riboflavin, chlorothiazide.
mucoadhesive dosage forms should be:
• not cause irritation
• small and flexible enough to be accepted by the patient.
These requirements can be met by using hydrogels.
Hydrogels : are hydrophilic matrices that are capable of
swelling when placed in aqueous media.
• As water is absorbed into hydrogels, chain relaxation
occurs and drug molecules are released through the spaces
or channels within the hydrogel network.
• Hydrogels matrices include natural gums and cellulose
derivatives.
Mechanism of mucoadhesion
The major constituents of mucus is mucin are the high molecular
weight glycoproteins, Mucin also has different charge density
Depending on the pH.
 For a good bioadhesive hydrogel , such as polycarbophil, the
penetration into the mucus layer is dependent on the initial
applied pressure.
 A moderately bioadhesive hydrogel, like polymethacrylate, shows
a capability to entangle with the mucus layer.
 A poor bioadhesive hydrogel, like polyhydroxy ethyl methacrylate
(PHEMA), shows little penetration into the mucus layer.
The interaction of mucoadhesive hydrogels with the mucus layer
Theories of mucoadhesion, depending on the chemical
nature of adhesive/adherent combinations :
1. Diffusion Theory:
The diffusion theory describes interpenetration of the
mucoadhesive
(polymer) and
substrate (mucin)
to a
sufficient depth and creation of a semipermanent adhesive
bond by physical entanglement which is dependent on the
molecular weight of the polymer and flexibility and chain
segment mobility of the mucoadhesive polymer
2. Adsorption Theory:
It is a surface force where surface molecules of adhesive
and adherent are in contact.
According to adsorption theory, bioadhesive systems adhere
to tissue due to bond formation.
* Primary Chemical Bonds
Many bioadhesives can form primary chemical covalent
bonds with functional chemical groups in mucin:
Aldehydes and alkylating agents can readily react with
amino groups and sulfhydryl groups.
Acylating agents react with amino and hydroxyl groups of
serine or tyrosine.
* Secondary chemical bonds:
Hydrogen bonding, electrostatic forces or Van-der Waals
attractions are sufficient to contribute adhesive joints.
3. Electronic Theory:
indicate
that
electronic
transfer
on
contact
of
the
bioadhesive polymer and the mucin glycoprotein, which lead
to the formation of a double layer of electrical charge at the
bioadhesive interface.
4. Wetting Theory:
the ability of the adhesive to spread on mucin influences the
contact
between
the
mucoadhesive
&
mucin,
that
consequently influences mucoadhesive strength. Thus work
of adhesion is a function of the surface tensions of surfaces
in contact, as well as the interfacial tension. A small
interfacial tension means more contact between the two
surfaces.
5. Mechanical Theory:
the adhesive flow into the pores and interstices to create
mechanical embedding embedded adhesive solidifies and
becomes inextractable. The mechanical theory depends on
irregularities of the surface and Highly fluid adhesives which
are able to penetrate into the cracks and crevices of the
adherent create mechanical embedding.
To generalize the bioadhesion phenomenon:
The First Step: (wetting theory)
A close contact between the mucoadhesive and adherent
occur by a good wetting of the mucoadhesive surface
(mucin layer) and the swelling of the mucoadhesive
polymer with a sufficient spreading to assure a contact at
the molecular level between the mucoadhesive and the
membrane.
The Second Step: (Mechanical and diffusion Theories)
Once
contact
is
established,
penetration
of
the
mucoadhesive into the crevices of the tissue surface.
Hydrated polymer chains are free to move and stretch and
become entangled or twisted when become into close
contact with the substrate.
The Third Step: (Adsorption and Electronic Theories)
Once entangled is established, the bioadhesives match their
active adhesive sites with those on the substrate to form an
adhesive bond; or the free entangled molecules form
cohesive bonds.
Evaluation Of Bioadhesive Properties
The test methods for bioadhesion measurements can be
classified into two categories:
A. In-Vitro (Ex-Vivo) methods:
1. Detachment force
2. Detachment weight method
3. Wash-off test
4. Electrical Conductance
B. In- Vivo methods
1. X-ray photography
2. Scintigraphic method
A. In-Vitro (Ex-Vivo) methods
1. Detachment force:
It is useful technique for adhesive characterization of
bioadhesive solid and semisolid dosage forms.
The adhesive force is determined by the work to break
adhesive extensions off the adhesive mass.
 test using one tissue layer was used for the bioadhesive
characterization of solid dosage forms
test using two tissue layers was used for the bioadhesive
characterization of semisolid dosage forms.
penetrometer, Texture Analyzer, can
be used.
bioadhesive
performance
determined
by
resistance
to
represent
the
was
measuring
withdraw
work
the
the
probe
required
for
detachment of the two systems.
TA-XT2i-Texture Analyzer
2. Detachment weight method:
Bioadhesive force is determined by the
following equation:
Detachment stress (dyne/cm2) x 102 = m * g / A
Where:
m = The minimal weight added to the balance cause detach (g).
g = Acceleration due to gravity (980 cm/sec 2).
A = Area of tissue exposed (π r2 ).
Force of adhesion (N)= Bioadhesive strength X 9.81
1000
3. Wash-off test:
•The method is used for the evaluation of mucoadhesion
properties of microparticles
Pieces of mucosal tissue were mounted onto glass slide.
About 100 microparticles, with mucoadhesive polymer are
spread onto wet tissue specimen. hold onto the arm of a USP
tablet-disintegration tester, permitting a slow, regular up
and down movement (30 min) in a test fluid kept at 37°C.
Mucoadhesive force of the tested polymer= Resistant to
hydrodynamic shear
4. Electrical Conductance:
• In the presence of adhesive material, the conductance was
comparatively low.
•As the adhesive was removed, the value increased to a
maximum value corresponding to the conductance of the
saliva, which indicated the absence of adhesion.
B. In- Vivo methods:
Based on the measurement of the residence time of
bioadhesives at the application site.
1. X-ray photography:
Barium sulfate (BaSO4) matrix dosage form, containing the
polymer whose bioadhesive properties want to be tested,
can be administered to the volunteer, who is subjected to X-
ray
studies.
X-ray
photographs
show
the
mucoadhesion of the polymeric dosage form.
extent
of
2. Scintigraphic method
The gastrointestinal transit times of bioadhesives have been
examined using radioisotopes as
55Cr-labeled
bioadhesive
material was inserted in the stomach and the radioactivity
was measured at time intervals.
FACTORS INFLUENCING BIOADHESION
Nature of Polymer:
Hydration of adhesives
Flexibility of adhesives
Molecular weight and size of adhesives
Function Groups of Adhesives
Charge Sign of the Adhesives
Charge density of adhesives
Physiological Variables:
Hydration of Biological Substrates
Turnover of Adherent
Nature of Surrounding Media :
pH of the Surrounding Media
Nature of Polymer:
A polymer characteristics are necessary for mucoadhesion:
(i)
Strong hydrogen-bonding group (-OH, -COOH).
(ii) Strong ionic charges.
(iii) High molecular weight.
(iv) Sufficient chain flexibility.
(v) Surface energy properties favoring spreading onto mucus.
Hydration of Adhesives
Many
hydrocolloids,
such
as
vegetable
gums
and
hydrogels, such as polycarbophil become adhesive after
hydration.
 Swelling
state is an important factor for adhesiveness
where the swollen polymer allows the relaxation of the
molecules, exposing their adhesive sites and facilitating
interpenetration to a sufficient depth in order to create
adhesive bonds.
However,
there is an optimum water concentration for
the hydrocolloid particles to develop maximum adhesive
strength where excessive water may cause slippery
nonadhesive mucilage.
Flexibility of Adhesives
 There is a relationship between structure and adhesion
of mucoadhesive polymers where bioadhesives should
possess optimal flexibility , to allow interpenetration of
polymer and mucus to take place, that permit the adhesive
to conform to the adherent.
 The flexibility of a polymer backbone is influenced by the
steric effect of substituent side groups.
As the size of the substituted side group becomes larger,
chain flexibility is decreased.
If
side
chains
are
flexible,
they
give
internal
plasticization to the whole polymer structure with
suitable adhesiveness.
Increased
cross-linking reduces chain flexibility that
decreases bioadhesive performance
As
acrylic acid hydrogels (as Carbopol) contain coiled
macromolecules, unable to form an elastic polymer
network as a result of the repulsion of negative charges
and many of the adhesively active groups are shielded
inside the coils and do not actively participate in the
adhesion process.
Thus, it is necessary to neutralize the produced anionic
liquid gels to help in the formation of an expanded gel
network.
 triethanolamine was preferred as neutralizing agent,
since relatively higher viscosity could be obtained using
organic amines than using inorganic bases (as sod.
Hydroxide) where cations generated by amines, resulting in
greater steric expansion of the polymer molecules than the
smaller sodium cations which leads to lower hydration of
the polymer.
Molecular Weight and Size of Adhesives:
Higher molecular weight leads to higher cohesive strength
and reduces creep (move), due to the greater degree of
chain entanglement resulting from longer chains.
Adhesive
force increases as polymer molecular weight
increases, until a plateau value is reached.
• At higher than optimum molecular weight, adhesion may
be reduced due to reduced penetration of the adherent
surface by adhesive polymers due to their low mobility.
Function Groups of Adhesives:
 For
mucoadhesion
to occur, polymers must have
functional groups that are able to form hydrogen bonds,
that explaine the excellent performance of adhesives,
containing phenolic or aliphatic hydroxyl groups with
polar substrates.
 Also
charged
carboxylated
polyanions
potential bioadhesives for drug delivery.
are
good
Charge Sign of the Adhesives:
Polymers commonly used can be classified as following:
Non-ionic polymers; as Hydoxypropyl cellulose (HPC) and
hydoxypropyl methylcellulose (HPMC).
Polycationic polymers; as Chitosan.
Polyanionic polymers; as Polyacrylic acid (PAA)
derivatives, e.g., carbopols (CP) and polycarbophils
Cationic and anionic polymers bind more effectively with
the epithelium than the neutral polymers.
Positively
charged polymeric hydrogels have additional
molecular-attractive
forces
due
to
the
electrostatic
interactions with negatively charged mucosal surfaces
Also
anionic polymers with sulphate groups bind more
effectively than those with carboxylic groups.
Charge Density of Adhesives:
It explain the mechanism whereby negative charge
polymers can bind to a mucus surface of the same charge
sign by the increase in the number of carboxyl or sulfonate
groups on the surface, which cause increase in wettability.
The reason for the excellent bioadhesive property of
Polycarbophil or Carbopol is due to that, they are both
polyanions with high charge density.
Physiological Variables :
Hydration of Biological Substrates
Effective adhesion can only occur, when an adhesive and
adherent are brought into molecular contact.
The presence of water and other fluids on the surface of
adherent may prevent full effective interactions at appropriate
interfaces ,Due to greatest disruptive
effect of water on adhesive bonds
occur with polymer systems, which
depend primarily on hydrogen bonding.
The dehydration theory
of mucoadhesion
Turnover of Adherent:
Mucus covering epithelial cells in the GIT ,
Nasal or eye is continuously secreted and eliminated.
The continuous renewal of the adherent as in soft tissue
bioadhesion , allow failure of a strong adhesive bond.
Thus bioadhesives which bind to this mucus layer are
expected to be removed at the same time when mucin
turnover regardless of the adhesive strength.
Nature of Surrounding Media :
pH of the Surrounding Media:
 Mucous has a different charge density, depending on pH,
due to differences in dissociation of functional groups on the
carbohydrate & in the amino acids of polypeptide backbone.
 As the pH of the adherent medium increased, charge
repulsion is increase with decrease in adhesion.
 The absorption of water by a polymer and its swelling,
depends on the pH.
 The interaction of polycarbophil with intestinal tissue was
negligible compared to that with stomach tissue due to the
difference in pH.
Applications of bioadhesion
Mucoadhesion
Transmucosal routes of drug delivery (i.e., the mucosal
linings of the eyes, nasal, rectal, vaginal and buccal
cavities) offer advantages for systemic drug delivery
include:
1. Bypass of first pass effect,
2. avoidance
of
presystemic
gastrointestinal tract.
elimination
within
the
1. Buccal mucoadhesives
Advantages Of Buccal Adhesive Drug Delivery Systems
1. The mucosa is relatively permeable (4-4000) times greater
than that of the skin with a rich blood supply that render
buccal adhesive drug delivery systems gained interest in
systemic delivery of drugs undergoing hepatic first-pass
metabolism within the gastrointestinal tract.
2.
Drug can be easily applied and localized to the application site
and can be removed.
3.
Buccal cavity is highly acceptable by patients.
Disadvantages Of Buccal Adhesive Drug Delivery Systems
1. the environmental factors such as the exposure of the oral
mucosa to salivary flow, shearing forces of tongue
movement and swallowing which can act to displace and
wash away an adhering vehicle
There are considerable differences in
permeability
between
different
regions of the oral cavity, because of
the varied structures and functions of
the different oral mucosa.
The permeabilities of the oral mucosa
decrease in the order of
sublingual > buccal > palatal.
This rank order is based on the relative thickness and degree of
keratinization of these tissues, with the sublingual mucosa
being relatively thin and non-keratinized, the buccal thicker
and non-keratinized and the palatal intermediate in thickness
but keratinized.
Thus, oral cavity drug delivery is classified into:
(i) Sublingual delivery
 Which is systemic delivery of drugs
through the mucosal membranes lining
the floor of the mouth.

Give rapid absorption with acceptable
bioavailability of many drugs.
(ii) Local delivery
Drug delivery into the oral cavity has a number of
applications
including,
the
treatment
of
toothaches,
periodontal diseases, aphthous and dental stomatitis.
(iii) Buccal delivery,
which
is
drug
administration
through
membranes lining the cheeks (buccal mucosa).
the
mucosal
2. Oral Mucoadhesion
to localize a drug and increase its residence time at a
certain site in the GIT.
Oesophageal Mucoadhesion:
• Oesophageal
mucoadhesion is used for prolonged
retention of drugs within the oesophagus for treatment
of upper gastro-oesophageal disorders.
• Alginate solution can form a coat for localization of
drugs within the oesophageal tissue for prolonged
periods of time.
Gastric Mucoadhesion:
 Gastric residence of a conventional dosage form is typically
short and transit rapidly through the small intestine. This
diminish the extent of absorption of many drugs.
The Gastric mucoadhesive most commonly used system for
prolonged residence time in stomach to improve the efficacy
of antibiotics to penetrate through the gastric mucus layer in
cases of gastritis, gastric ulcer and gastric carcinoma due to
Helicobacter pylori.
Potential drug candidates for gastro-mucoadhesive :
a. Drugs that have absorption windows in the upper part of
the gastrointestinal tract.
b. All drugs that are intended for local action on the gastroduodenal wall, as in case of ulcerous diseases.

Carbomers and HPMC have good properties with the
gastric mucoadhesion.

Mucoadhesive chitosan microspheres interact with
sialic acid in the gastric mucus by electrostatic
interaction that improve the gastric residence time of a
drug. Also provide pH-responsive release profile by
swelling in acidic environment of the gastric fluid.
Intestinal Mucoadhesion:
Mucoadhesive microspheres applied into the intestine using
Chitosan as a cationic mucoadhesive polymers can resist
hydrodynamic
shear
leading
to
in
vivo
absorption
enhancement of orally administered drugs.
 Chitosan microspheres can be used for the oral delivery
of vaccines, based on its bioadhesive properties and
biodegradability.
 Polycarbophyl beads, as an anionic bioadhesive are
washed-off very rapidly.
Colon Mucoadhesion:
Colon mucoadhesion tablets remain intact in the stomach
due to the enteric coat (Eudragit®L100).
In small intestine, with alkaline pH, the enteric coat will
dissolve
 Upon entry into the colon, the azo-networks of HPMC
degrade by microbial azo reductase present in the colon to
produce a structure, capable of developing mucoadhesive
interactions with the colonic mucosa.
3. Rectal Bioadhesion:
Anatomically, the upper part of the rectal venous drainage
is connected with the portal system, while the lower part
directly with the general circulation.
 solid
suppository have hepatic first-pass elimination of
the drugs following rectal administration.

Liquid suppositories containing mucoadhesive polymers
were administered intrarectally to avoiding first-pass
hepatic
elimination
hepatotoxicity
ketoconazole.
of
of
the
some
drug
and
drugs
as
avoid
the
antifungal
 Mucoadhesive
polymers sodium alginate were added to
liquid suppository bases Poloxamers (pluronic 407 and
P 188) to exhibit great mucoadhesive characterization
with no irritation of the rectal mucosal membrane and
diminish the migration distance of the suppository in
rectum without leakage after administration.
4. Vaginal Bioadhesion:
 Vaginal delivery is useful for systemic drug absorption as
well as local action.
 A numbers of factors including changes in vaginal
environment cause some problems for drugs. Bioadhesive
systems of sodium alginate and
Chitosan may overcome
these problems by yielding safe vaginal delivery systems as
contraceptive vaginal formulations.
5. Transurethral Bioadhesion:
The most common treatment method for carcinoma of the
bladder is known as the transurethral resection (TUR).
 to obtain desired attachment onto the bladder wall for
pharmacotherapy after TUR, mucoadhesive chitosan carrier
was prepared in the form of cylindrical geometry.
6.Nasal Mucoadhesion
The nasal cavity can be used as a site
for systemic drug delivery.
 chronic
application
of
nasal
dosage
forms cause irreversible damage to the
ciliary action of the nasal cavity
 the
large
intra-
and
3
2
inter-subject
variability in mucus secretion of the nasal
1
mucosa, could significantly affect drug
absorption from this site.
1. Lower region for air way
2. Middle region for systemic way
3. Upper region for olfactory way
Advantages
 intranasal drug delivery is ease of administration
 rapid drug absorption
 avoidance of hepatic first-pass metabolism.
The richly supplied vascular nature of the nasal mucosa
with its high drug permeation, makes the nasal route of
administration attractive for many drugs.
The most efficient area for drug
absorption through nasal mucosa is
the lateral wall of the nasal cavity.
The
mucociliary
clearance
is
inversely related to the residence
time and the absorption of drugs
administered.
 A prolonged residence time in the
nasal cavity may be achieved by using
bioadhesive polymers, as chitosan
7. Pulmonary Bioadhesion (Airway Delivery):
Advantege:
 prolonging drug action and reducing drug dosage can be
ashieved by using Pulmonary Bioadhesion .
Methyl cellulose (MC), Sodium carboxy methylcellulose
(SCMC) Hydroxy propyl cellulose (HPC) are most commonly
used polymers.
Powder inhalation in airway path for
pulmonary bioadhesion
8. Ocular Bioadhesion:
 Drugs
administered
systemically
have poor access to the inside of the
eye, because of the blood-aqueous
and blood-retinal barriers.
 Topically
applied drugs are rapidly eliminated from the
precorneal area. lost within 15-30 sec. due to reflex tearing
and drainage via the nasolacrimal duct.
 The
cornea is considered as an effective barrier to drug
penetration, since the corneal epithelium has tight junctions
which completely surround and effectively seal the superficial
epithelial cell.
 Some
polymers have the capacity to adhere to the mucin
coat covering the conjunctiva and the corneal surface of
the eye prolonging the residence time of a drug.
 At
physiological pH of tears, the mucus network usually
carries a significant negative charge because of the
presence of sialic acid and sulfate residues.
Ophthalmic bioadhesives including hydrogels like
carbopols, polyacrylic acids and chitosan which can be
formulated as mucoadhesive erodible ocular inserts,
minitablets, microspheres or hydrogels.
9. Hemostasis and Wound Dressing Bioadhesion
Bioadhesives have been used as
haemostatic and wound healing agents.
Requirements for good Bioadhesive polymers
for haemostatic and wound healing.
Have the ability to spread on tissue surfaces
Must be rapidly and uniformly adhere and
conform to
wound bed topography and contour to prevent air or fluid
pocket formation.
Prevents
peripheral channeling into the wound by
bacteria and promotes bonding to tissues.
Must be Permeable to water vapor
to the extent, that moist exudates
under the dressing is maintained
without pooling, but excess fluid
absorption and evaporation leading
to desiccation of the wound bed.
Must
not interfere with normal
progress of natural repair process,
compatible with body tissues, be
nontoxic, non irritant, non-antigenic
and non-allergenic.
Fibrinogen and cyanoacrylates are
effective in face-to-face sealing of
tissues or wound healing.
Chitosan could be dissolved in organic acids, such as lactic
acid and acetic acid and casted into films forming soft,
flexible and pliable bioadhesive wound healing bandages
able to effectively bind and agglutinate a wide variety of
mammalian cell types.
Cross-linked gelatin films were bonded to heart muscle and
to
lung
pleura
and
parenchyma,
using
the
electrical
discharge of an argon beam radio-frequency coagulator.
denatured protein constituents of both gelatin and tissue
protein chains create a fluidized state that rapidly coalesced.
Dental Bioadhesion
 Adhesion to a tooth substance is difficult,
because the surface is not usually smooth and the external
enamel is coated with an organic proteinaceous cuticle.
 Dental adhesives, such as polyacrylic acid, to enamel
explained by the ability of free carboxyl groups to displace
phosphate ions from the apatite matrix to ensure excellent
wetting.
 Materials that adher to calcified tissue forming chelate
with calcium as poly (acrylic acid) considered as good dental
adhesives.
Transdermal bioadhesion
Transdermal bioadhesion (drug-in-glue patch- systems)
Advantages of Transdermal delivery:
Reduce the systemic toxicity and side effect.
Minimize the loss of drug, due to first pass metabolism
 Gastrointestinal adverse effects can be avoided
Easily termination of therapy
Release of the drug is controllable
 The
bonding strength of
glutaraldehyde crosslinked
gelatin films with biological
tissue is due to aldehyde in
the GA-gelatin films and
the amino groups of the
natural tissue.
 Organogels obtained by adding small amounts of water
to organic solution of lecithin produce lecithin gels as
efficient bioadhesive vehicles for transdermal transport
of various drugs