Transcript 06_Gamboa_Jessica - Arizona Space Grant Consortium

Plant-Based
Biopharmaceutical Production
under Controlled
Environments
Jessica Gamboa- University of Arizona
Biosystems Engineering
Chieri Kubota, Ryo Matsuda, Lucrecia M.
Alvarez, & Guy A Cardineau
Our Eventual Goal
Use plant made pharmaceuticals to produce
an easily deliverable form of the vaccine for
the plague (Y. pestis)
This will be done using plant-made
pharmaceuticals grown under
a controlled environment
Plague
Plague is an infectious disease that is
caused by the bacterium Yersinia
pestis, it can be deadly.
The F1-V protein is used as an
antigen fusion protein against plague.
Experimental Goal
To quantify…
plant growth
development
photosynthesis
total soluble protein concentration
The tomato plants were
modified to produce edible
F1-V protein, a form of the
vaccine for the plague
Greenhouse growth is
advantageous because high
production and containment
is achievable
Items measured in experiment
Stem growth
Leaf production
Light-saturated rate of photosynthesis
in leaves
Weekly fruit yield
Total soluble-protein concentration
F1-V concentration in fruits
Plants Grown in Greenhouse
‘Durinta’ - Commercial greenhouse
cultivator
‘TA234’ - Wild Type
‘T22.1’ - Transgenic line
‘T3D1.2’ - Supertransformed line
Plant Growth and Leaf
Production
Stem Length
‘Durinta’>‘T22.1’=‘TA234’= ‘T3D1.2’
Number of Leaves
‘Durinta’= ‘T22.1’ =‘TA234’=‘T3D1.2’
Weekly Stem Elongation Averages
35
Centimeters
30
25
20
15
10
5
0
Durinta
T22.11
T3D1.2
TA234
Weekly Leaf Development
Number of Leaves
4.5
4
3.5
3
2.5
2
1.5
1
0.5
0
Durinta
T22.11
T3D1.2
TA234
Photosynthesis, Fruit Yield
and Total Soluble Protein
Light-saturated rate of photosynthesis
‘Durinta’ ≥ ‘T22.1’ = ‘TA234’ = ‘T3D1.2’
Fruit Yield
‘Durinta’ > ‘TA234’ > ‘T22.1’ =‘T3D1.2’
Total Soluble Protein in Green Fruits
‘T22.1’ > ‘T3D1.2’ > ‘TA234’ >‘Durinta’
Conclusion
Transgenic tomato plants showed a lower
growth rate and lower yield, but produced
more protein than ‘Durinta’, the commercial
greenhouse standard.
Transgenic tomato plants are considered
promising candidates for high value protein
production.