Transcript Parts of the Microscope and Their Function

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The invention of the microscope made it
possible for people to discover and learn
about cells.
A microscope is an instrument that makes
small objects look larger.
The ability to make an
object look larger than
it is through the bending
of light that passes
through lenses.
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Resolution is the ability to clearly distinguish
the individual parts of an object.
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The light microscope. The common light microscope used in the
laboratory is called a compound microscope because it contains two
types of lenses that function to magnify an object.
The more traditional form of electron microscope is the transmission
electron microscope (TEM). To use this instrument, one places ultrathin
slices of microorganisms or viruses on a wire grid and then stains them
with gold or palladium before viewing. The densely coated parts of the
specimen deflect the electron beam, and both dark and light areas show
up on the image.
The scanning electron microscope (SEM) is the more contemporary
form electron microscope. Although this microscope gives lower
magnifications than the TEM, the SEM permits three-dimensional views
of microorganisms and other objects. Whole objects are used, and gold
or palladium staining is employed.
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#9 Eye Piece/Ocular Lense
The part you look at with
your eye. Usually 10 X
magnification.
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#10 Arm – Used to safely
transport microscope
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#11 Stage – Slides are
placed on this
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#12 Coarse Adjustment Knob
– Used to make large
changes in focus. NOTE
Never use this when viewing
on high power
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#13 Fine Adjustment Knob
Used to small adjustments
of focus
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#14 Base – Used to safely
transport the microscope
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#1 Body Tube – Reflects
light up to the viewers eye
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#2 Nosepiece– Allows for
quick change of objectives
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#3 Low Power Objective –
The first lens you use when
doing proper microscope
work. Usually 4 X
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#4 Medium Power
Objective – The second
lens you use when doing
proper microscope work.
Usually 10 X
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#5 High Power Objective – The
highest magnification used.
Usually 43 X. NEVER use the
course adjustment when using
this lens.
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#6 Stage Clips – Use to
keep the slide in place.
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#7 Diaphragm – Use to vary the
amount of light passing through
the slide. Usually it is better if
the amount of light is low.
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#8 Light Source – Sends
light up through the
diaphragm and through the
slide for viewing
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Carry MICROSCOPE by arm and base.
Always lower the stage BEFORE you move
the objective lenses.
Always have a cover slip over the specimen
you are looking at.
If you are looking at cells that are translucent
you have to stain them with methylene blue
or iodine.
Report any incidents to teacher!