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Phlebotomy: Venipuncture
Phlebotomy
What does it mean?
Process of collecting blood
Dates back to ancient Egypt
Bloodletting-”bad” blood
Greek translation
Phlebos- vein
Tome –incision
Why collect blood?
Diagnosis and treatment of disease
Therapeutic purposes
Blood donation
The Medical Assistant’s Role
Perform venipuncture accurately and
efficiently
Reliable test results
Provide quality care
Patient safety and confidentiality
Phlebotomist safety (PPE)
Anatomy and Physiology of the Circulatory
System
Blood forms in body’s organs and
bone marrow
Blood carries oxygen and nutrients
to cells and removes waste products
and carbon dioxide
(Review your terminology textbook Hematology and
Immunology)
Do you know?
The difference between an
artery and a vein?
Circulatory System Cont’d
Arteries and Veins
Arteries
Carry oxygenated
blood away from the
heart
Pulse
Thick walls
No Valves
Veins
Carry deoxygenated
blood to the heart
No pulse
Thin elastic walls
Valves
Circulatory System Cont’d
Body contains about 6 liters of blood
Forty-five percent of blood is formed elements
Erythrocytes
Leukocytes
Thrombocytes (platelets)
Two mL of blood will yield about 1 mL of fluid
Liquid part of blood is plasma
Important Terms
Fibrinogen –substance in blood Converted
by the blood clotting process to fibrin
Fibrin- “sticky” web like substance –traps
the formed elements-result is a “blood clot”
Serum- clear liquid portion of blood
extracted from the “blood clot” used for
many tests
Important Terms
Anticoagulant-used to prevent blood
from clotting. Found in certain blood
tubes
Buffy coat-layer separating plasma
from RB
Centrifuge- machine which spins
blood separating the RBC from the
serum
Anatomy and Physiology
of the Circulatory System
Centrifuge separates serum from blood
Anticoagulant prevents blood from clotting to produce
plasma specimen
Buffy coat
Phlebotomy Sites
Order of Draw by sites
Arm-Antecubital Space includes
Median cubital
Cephalic
Basilic
Veins in hand
Veins in feet
Preferred Sites in the arm
Arteries of Arm
Note locations of arteries in the
arm. You DO NOT want to draw
from an artery
Brachial
Radial
Ulnar
How can you distinguish an artery from a
vein?
Venipuncture Methods
There are three main methods of
drawing blood
Vacuum Tube
Syringe
Butterfly
Evacuated Tube Method
This method includes:
Vacutainer tube
Adapter
Double-pointed Needle
Features of this method are:
Closed system - needle stick risk low
Vacuum draws blood inside the tube
Safety needles meet OSHA guidelines
Venipuncture Technique
Butterfly collection system
Combines benefits of syringe and vacuum methods
Used for small or fragile veins that are difficult to draw
Winged needle inserted at about 5° angle then threaded
into vein
Syringe method
Used for small or fragile veins
Control flow
Venipuncture Equipment
Safety needles
Required
Protects patient and health care worker
Equipment/Supplies Needed
Physician order and Lab requisition form
Pen –black ink
Appropriate PPE (gloves, gown, goggles)
Needle holder and Needle (varying sizes)
Tubes –varying sizes and types used
More Equipment/Supplies
Tourniquet or blood pressure cuff
Constrict flow of blood in arm
Make veins more prominent
Alcohol or Betadine
Cotton balls or gauze-(preferred)
Bandages or tape
Order of draw
Why? Prevent carryover or contamination - Order may
vary between laboratories.
Basic order:
Sterile- sterile specimens
Light blue-sodium citrate
Red or plain tubes no additives or gel
SST –Serum separator tube (Red/Gray, Yellow)
Green –heparin
Lavender-EDTA
Gray –oxalate-fluoride
Venipuncture Equipment
Additives, anticoagulants, and gels
Thixotropic separator gel >>
Separator gel tube: centrifuging process
Glass particles activate clotting
Venipuncture Equipment
Specimen collection trays
Hold all equipment necessary
Carts also used
Venipuncture Technique
A Phlebotomist needs to use all these skills:
Technical skills
Prepare for the draw-equipment (spare tubes)
Positioning the patient
Administrative skills
Note order-required tests and
Complete the lab requisition
Social skills
Small talk (weather, pets, entertainment)
Good Therapeutic Communication
Assess patient comfort level
Put patient at ease-show confidence
Ask about past experiences
Preparing the patient
Identify yourself and explain the procedure
Properly Identify your patient ask them to:
State their name and date of birth
For non-English speaking photo ID
Note fasting or non-fasting
Ask patient about allergies
Taking blood thinners
Past Experiences
Remember to
Wash your hands
The Draw
Position your patient for safety:
sit
lie down
(note past history of fainting or loss of consciousness)
Select the appropriate site and vein free of:
Scars
Hematomas
A Pulse
The Draw Cont’d
Find site that will give best blood return
(remember the location order)
Palpate vein with tip of finger
Differentiating between veins, tendons,
and nerves
Use the tourniquet appropriately
Proper Tourniquet Technique
To prevent inaccurate blood test results-do
not leave on longer than one minute
Tourniquet may be applied over clothing for
patient comfort
Remove tourniquet when blood flow is
achieved or prior to inserting last tube
Always remove tourniquet prior to removing
needle
Proper completion of draw
Remove tourniquet
Remove needle and apply safety device while Applying
pressure to site (patient can assist)
Bandage appropriately
Tend to the safety of your patient
Label specimen tubes in front of patient
Venipuncture Technique
Patient and specimen
identification
Computer label >>
Compare lab requisition
with patients information
Important Tips
Do Not draw from a resistant patient
Do Not draw if you are not comfortable
Uncooperative children must receive special
care and be restrained for safety
Note Patient reactions, stop if patient
complains of pain-assess patient
Venipuncture Technique
The unsuccessful venipuncture
Reposition tube and needle
Rotate needle Advance further into vein
Try another tube-maybe the tube is
defective
If you haven’t succeeded in getting blood
after 2 tries ask someone else to try
Venipuncture Technique
Factors affecting laboratory values
Incorrect specimen handling and storage
Drawing procedure incorrect
Alcohol in specimen
Tourniquet on too long
Wrong tube
Tubes not filled to appropriate levels will cause
inaccurate test results
Patient Factors Affecting Lab Results
Blood Alcohol-elevate
Hemolysis-destroys RBC’s
results
Diurnal rhythm-RX or daily
activity level
Exercise-runner’s anemia,
change results
Fasting (not)-inaccurate
results
Heparin-incorrect use
interferes with results
Stress-Increase in WBC’s
Tourniquet on too longdilution hemoconcentration
Challenging Patients
Pediatric
The child, parents or guardians
Approach-explain –get down to their level
Resistant-restraints ?
Geriatric
Physical-skin, hearing or vision impaired
Effects of disease-stroke, arthritis, Parkinson’s –tremors,
Dementia-Alzheimer's
Safety issues-wheelchairs, balance
More Challenges
Dialysis or Cancer patients -fistulas and
shunts
(AV-arteriovenous-permanent access for
dialysis)
Long-Term Care patients, Home Care,
or Hospice Patients
Traveling phlebotomists
Problem Sites
Burns, Scars, Tattoos
Damaged Veins (sclerosed-thrombosed)
Edema-swelling due to abnormal
accumulation of fluid
Hematoma-swelling or mass of blood
Mastectomy-caution lymph node removal
Obesity
Complications or Conditions
Allergies-adhesive, antiseptic, latex
Excessive bleeding due to medications, hemophilia
Fainting
Nausea and vomiting
Pain while drawing-what is normal –what is NOTstopping the draw
Seizures/Convulsions-stop draw immediately-get
help
Comprehensive Medical Assisting 4th Edition
Clinical Medical Assisting: A professional, Field
Smart Approach to the Workplace, Textbook and
Workbook
Phlebotomy Essentials 4th Edition