Pneumocystis carinii
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Transcript Pneumocystis carinii
Unit 14 Infection Control
14:1 Understanding the
Principles of Infection Control
Understanding is essential to all health
care workers
Provide a basic knowledge of how disease
is transmitted
Main emphasis on prevention of disease
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2
Microorganisms or
Microbes
Small living organisms
Not visible to the naked eye
Microscope must be used to see them
Found everywhere in the environment
Found on and in the human body
Many are part of normal flora of body
May be beneficial
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3
Microorganisms or
Microbes (continued)
Called nonpathogens when not harmful to
the body
Some cause infections and disease
Called pathogens (germs) when able to
harm the body
Sometimes nonpathogenic
microorganisms can become pathogenic
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4
Microbe Classifications
Bacteria
Protozoa
Fungi
Rickettsiae
Viruses
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5
Bacteria
Simple, one-celled organisms
Multiply rapidly
Classified by shape and arrangement
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6
Bacteria – Cocci
Round or spherical in shape
Diplococci
Streptococci
Staphylococci
Examples of diseases
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Bacteria – Bacilli
Rod-shaped
Occur singly, in pairs, or in chains
May have flagella
Ability to form spores
Examples of diseases
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8
Bacteria – Spirilla
Spiral or corkscrew shape
Includes comma-shaped vibrio and
corkscrew spirochete
Diseases include syphilis and cholera
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9
Antibiotics
Antibiotics are used to kill bacteria
Some strains of bacteria have become
antibiotic-resistant
When antibiotic-resistant, the antibiotic is
no longer effective against the bacteria
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10
Protozoa
One-celled, animal-like organism
Found in decayed materials and
contaminated water
May have flagella for movement
Some are pathogenic
Examples of diseases
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11
Fungi
Simple, plant-like organisms
Live on dead organic matter
Yeast and molds
Can be pathogenic
Examples of diseases
Antibiotics do not kill
Antifungal medications
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12
Rickettsiae
Parasitic microorganisms
Cannot live outside the cells of another
living organism
Transmitted to humans by the bites of
insects (e.g., fleas, lice, ticks, mites)
Examples of diseases
Antibiotics are effective against
many of them
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13
Viruses
Smallest microorganisms
Must use electron microscope to see
Must be inside another living cell
to reproduce
Spread by blood and body secretions
Very difficult to kill
Cause many diseases
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14
Virus – Hepatitis B
Also called serum hepatitis
Caused by HBV
Transmitted by blood serum and
body secretions
Affects the liver
Vaccine available for protection
Vaccine is expensive
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15
Virus – Hepatitis B
(continued)
Vaccine given in a series of
three injections
By law, employers must provide vaccine at
no cost to employees with occupational
exposure to blood or other body secretions
If employee refuses, a written statement
must be signed documenting refusal
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16
Hepatitis C
Caused by HVC
Transmitted by blood and blood-containing
body fluids
Many infected individuals
are asymptomatic
Others have mild symptoms
Can cause severe liver damage
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17
Hepatitis C
(continued)
Currently, no vaccine ready for use
Vaccine is in development stage
Extremely difficult to destroy HVC
Can survive several days in dried blood
Health care workers must follow
precautions to protect against virus
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18
Acquired Immune Deficiency
Syndrome (AIDS)
Caused by the Human Immunodeficiency
Virus (HIV )
Suppresses the immune system
Individual becomes susceptible to cancers
and infections that would not affect a
healthy person
No cure presently and no vaccine
Important to take precautions to prevent
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19
Growth of
Microorganisms
Most prefer warm environments
Most prefer darkness
Need source of food and moisture
Need for oxygen varies
Human body is ideal supplier of all
the requirements
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How Pathogens Cause
Infection and Disease
Some produce poisons called toxins
Some cause an allergic reaction
Others attach and destroy the living cells
they invade
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21
Classifications of
Diseases and Infections
Endogenous
Exogenous
Nosocomial
Opportunistic
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Endogenous
Originates within the body
Examples: metabolic disorders, congenital
abnormalities, tumors, and infections
caused by microorganisms within the body
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23
Exogenous
Originates outside the body
Examples: radiation, chemical agents,
trauma, electric shock, and
temperature extremes
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Nosocomial
Acquired in a health care facility
Usually present in the facility and carried
by health care workers to the patient
Many are antibiotic-resistant
Can cause serious and even
life-threatening infections
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25
Nosocomial
(continued)
Common examples are staphylococcus,
pseudomonas, enterococci
Infection control programs are used to
prevent and deal with
nosocomial infections
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Opportunistic
Infections that occur when the body’s
defenses are down
Usually do not occur in normal
immune system
Examples: Kaposi’s sarcoma (rare type of
cancer) and Pneumocystis carinii
pneumonia in individuals with AIDS
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27
Chain of Infection
Must be present for disease to occur and
spread from one individual to another
– Causative agent
– Reservoir
– Portal of exit
– Mode of transmission
– Portal of entry
– Susceptible host
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Causative Agent
Pathogen must be present
Examples include bacteria or virus
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Reservoir
Place where causative agent can live
Examples: human body, animals,
and the environment
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Portal of Exit
Way for causative agent to escape from
the reservoir
Examples: urine, feces, saliva, blood,
tears, mucous discharge, sexual
secretions, and draining wounds
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31
Mode of Transmission
Must be transmitted to another reservoir or
host where it can live
Direct contact: person-to-person, spread
by physical or sexual contact
Indirect contact: from contaminated
substances to the person
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Portal of Entry
Way to enter a new reservoir or host
Examples: breaks in the skin or
mucous membranes, respiratory tract,
digestive tract, genitourinary tract,
and circulatory system
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Susceptible Host
Individual who can contract the disease
Usually the person can fight off
the causative agent and not contract
the disease
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Common Body Defenses
Mucous membranes
Cilia
Coughing and sneezing
HCL in the stomach
Tears
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Common Body Defenses
(continued)
Fever
Inflammation response – leukocytes
Immune response – antibodies and
cell secretion
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Increased Susceptibility
When large numbers of pathogens
invade the body
When body defenses are weak
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Ending the Chain
of Infection
Eliminate any step in the chain and
infection is stopped
Follow practices to interrupt or
break the chain
Remember, pathogens are everywhere
Prevention is a continuous process
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Aseptic Techniques
Major way to break the chain of infection
Asepsis: absence of disease-producing
microorganisms or pathogens
Contaminated: any object or area that may
contain pathogens
Major aim: maintaining cleanliness and
eliminating or preventing contamination
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Common Aseptic Techniques
Handwashing
Good personal hygiene
Disposable gloves
Proper cleaning of instruments and
equipment
Thorough cleaning of environment
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Levels of Aseptic Control
Antisepsis
Disinfection
Sterilization
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Antisepsis
Prevent or inhibit the growth of
pathogenic organisms
Usually not effective against spores
and viruses
Can usually be used on the skin
Examples: alcohol and betadine
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42
Disinfection
Destroys or kills pathogenic organisms
Not always effective against spores
and viruses
Chemical disinfectants are used
Can irritate or damage the skin, so mainly
used on objects, not people
Examples: bleach solutions and zephirin
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Sterilization
Destroys all microorganisms
Steam under pressure, gas, radiation,
and chemicals
Autoclave is the most common
equipment used
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Summary
Important for health care workers to know
and use proper aseptic techniques
Prevents spread and transmission
of disease
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14:2 Washing Hands
Major aspect of standard precautions
Most important aseptic technique
Hands are perfect media for the spread
of pathogens
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Purpose of Handwashing
Prevent and control spread of pathogens
Protect the health care worker from
disease and illness
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When to Wash Hands
When arrive at facility
Immediately before leaving facility
Before and after every patient contact
Anytime the hands become contaminated
during a procedure
Before applying gloves
Immediately after removing gloves
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When to Wash Hands (continued)
Before and after handling any specimen
After contact with any soiled or
contaminated item
After picking up any item off the floor
After personal use of the bathroom
After you cough, sneeze, or use a tissue
Before and after any contact with mouth or
mucous membranes
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Principles of Handwashing
Use soap as a cleansing agent
Use warm water
Use friction
Clean all surfaces
Point fingertips down while washing hands
Use dry paper towels to turn faucet
on and off
Clean nails
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14:3 Observing Standard
Precautions
Blood and body fluids are the main ways
pathogens are spread
Major pathogens: HBV, HBC, HIV
Extreme care must be taken at all times
when an area, object, or person is
contaminated with blood or body fluids
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Bloodborne Pathogen
Standards
Established in 1991 by OSHA
Must be followed by all
health care workers
Civil penalties if not implemented
and followed
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Regulation Requirements
Written Exposure Control Plan
Identify all employees with
occupational exposure
Provide hepatitis B vaccines free of charge
Provide personal protective equipment
(PPE)
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Regulation Requirements
(continued)
Provide adequate handwashing facilities
and supplies
Ensure worksite is maintained in a clean,
sanitary condition
Follow measures for immediate
decontamination of surfaces
when contaminated
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Regulation Requirements
(continued)
Dispose of infectious waste correctly
Enforce rules of limited activities in any
potentially contaminated area
Provide appropriate containers
for contaminated sharps and other
contaminated items
(color coded and labeled)
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Regulation Requirements
(continued)
Post signs at the entrance to work areas
with occupational exposure to
biohazardous materials
Provide a confidential medical evaluation
and follow-up for any employee who has
an exposure incident
Provide training to employees
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Needlestick Safety
and Prevention Act
Passed by Congress in 2000
Centers for Disease Control and
Prevention (CDC) estimated 600,000 to
800,000 needle sticks occur each year
OSHA revised Bloodborne Pathogen
Standard to requirements of this act
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Employer Requirements
Identify and use effective and safer
medical devices
Incorporate changes in annual update of
Exposure Control Plan
Solicit input from nonmanagerial
employees who are responsible for direct
patient care
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Employer Requirements
(continued)
Maintain a sharps injury log
Ensure that every employee uses
standard precautions at all times
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Standard Precautions
Rules developed by the CDC
Every body fluid must be considered
potential source of infection
All patients must be considered potential
source of infection
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When to Use
Standard Precautions
Any situation where you might come in
contact with
– Blood or any fluid that contains blood
– Body fluids, secretions, and excretions
– Mucous membranes
– Nonintact skin
– Tissue or cell specimens
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Gloves
Gloves must be changed after contact with
each patient
When removing gloves, do not
contaminate your skin
Hands must be washed immediately after
removal of gloves
Gloves must not be reused
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When to Wear Gloves
Whenever contact with body fluids,
secretions, or excretions
When handling or cleaning contaminated
items or surfaces
Performing any invasive procedure
Performing venipuncture or blood tests
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Gowns
Worn during procedures that may cause
splashing or spraying
Helps prevent contamination of clothing
or uniforms
Contaminated gowns must be handled
per policy
Hands must be washed immediately after
removing gown
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Masks and Protective Eyewear
or Face Shields
Worn during procedures that may cause
splashing or spraying
Prevents exposure of the mucous
membranes of the mouth, nose, and eyes
Masks are used once and discarded –
change every 30 minutes following correct
procedure and immediate handwashing
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Masks and Protective Eyewear
or Face Shields (continued)
Protective eyewear and face shields
protect front, top, bottom, and sides
of eyes
If not disposable, must be cleaned and
disinfected before reuse
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Sharp Objects
Use extreme caution to avoid cuts
or punctures
When possible, use safe needles or
needleless system
Follow policies regarding handling needles
Use sharps containers
Follow laws regarding disposal of sharps
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Spills and Splashes
Wipe up immediately
Wear gloves when wiping up
Use disposable cleaning clothes
Use disinfectant with 10% bleach solution
Clean all contaminated surfaces
For large spills, can use absorbent powder
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Resuscitation Devices
Use to avoid the need for mouth-to-mouth
resuscitation, whenever possible
Place in convenient location that is
readily accessible
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Waste and Soiled Linen
Wear gloves
Follow agency policy
Use biohazard bags appropriately
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Reporting Cuts and Injuries
Report any cut or injury,
needle stick, or splashing of blood or
body fluids immediately
Follow agency policy
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Summary
Standard precautions must be followed at
all times by all health care workers
Observing these precautions can help
break the chain of infection
Allow health care workers to protect
themselves, their patients, and all
other individuals
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14:4 Sterilizing with
an Autoclave
Equipment that uses steam under
pressure or gas
Most effective method of sterilization
Available in various sizes and types
Preparation of equipment or supplies
Wrapping items for autoclaving
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Sterilizing with
an Autoclave (continued)
Autoclave indicators
Loading the autoclave
Time period for sterilization
Care of items after autoclaving
Dry heat sterilization
Follow directions on specific autoclave
Follow agency policy for sterile supplies
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14:5 Using Chemicals
for Disinfection
May not kill spores and viruses
Disinfect, but do not sterilize
Used to disinfect instruments that do not
penetrate body
Preparation of items
Chemical solutions used
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Using Chemicals for
Disinfection (continued)
Read manufacturer’s instructions
Antirust tablets or solutions added at times
Requirements for chemical disinfection
Chemical must completely cover items
Care of chemical solutions
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13:6 Cleaning with an
Ultrasonic Unit
Uses sound waves for cleaning
Method of aseptic control
Only ultrasonic solutions should be
used in unit
Permanent tank of ultrasonic unit
Items cleaned in ultrasonic unit
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Cleaning with an
Ultrasonic Unit (continued)
Care of glass beakers
Care of the permanent tank
Read manufacturer’s instructions before
using any ultrasonic unit
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78
Summary
Different methods provide different levels
of aseptic control
Method used depends on
equipment available and level of
aseptic control required
Read manufacturer’s instructions
Clean, rinse, and dry all equipment
before processing
Handle items carefully after processing
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79
14:7 Using Sterile Techniques
Many procedures require use of
sterile techniques to protect a patient
from infection
Surgical asepsis keeps an object or area
free from living organisms
Sterile: free from all organisms
Contaminated: organisms and
pathogens present
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Using Sterile
Techniques (continued)
Important to differentiate between sterile
and contaminated areas or items while
using sterile technique
Correct techniques must be
strictly followed to maintain sterility and
prevent contamination
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81
Using Sterile
Techniques (continued)
Clean working area required
Handling of sterile supplies
Sterile field: area used for placement of
sterile supplies
Rules of working within sterile area
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82
Removing Articles from
Sterile Wraps
Drop technique
Mitten technique
Transfer forceps
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83
Wet Surfaces
Organisms and pathogens travel quickly
through wet surfaces
If sterile item gets wet, contamination
has occurred
Use extreme care while pouring solutions
into sterile bowls
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Sterile Gloves
Worn while performing sterile technique
Make sure tray is open and all sterile items
are ready before putting sterile gloves on
your hands
Are sterile on the outside and
contaminated on the inside
Observe correct technique when putting
on sterile gloves
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85
Sterile Gloves
(continued)
When gloves are on, hold hands away
from body and above waist
Handle only sterile objects when wearing
sterile gloves
Change gloves any time
contamination occurs
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86
Sterile Dressings
Obtain proper authorization
Use an infectious waste bag
Wear disposable gloves for removing
a dressing
Note type, color, and amount of drainage
on dressing
Put on sterile gloves to cleanse area and
apply new dressing
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87
Sterile Dressings
(continued)
Cleanse wound or incision with
correct technique
Apply inner and outer dressing, taking
care to maintain sterility of dressings
Remove sterile gloves properly and
dispose of properly
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88
Sterile Dressings
(continued)
Apply tape correctly to hold dressing
in place
If contamination occurs at any time during
the procedure, start over
Commercially prepared sterile supplies are
widely available
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13:8 Maintaining TransmissionBased Isolation Precautions
Some diseases are communicable –
caused by organisms that can be
transmitted easily
These precautions are in addition to the
Standard Precautions
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Maintaining Transmission-Based
Isolation Precautions (continued)
Help prevent spread of disease to others
Protects patient, family, and
health care workers
Type used depends on the causative
organism of the disease
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91
How Communicable
Diseases Spread
Direct contact with a patient
Contact with dirty linen, equipment,
and supplies
Contact with blood, body fluids, secretions,
and excretions
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Terms Defined
Contaminated or dirty: items that contain
disease-producing organisms; must not be
touched unless protected
Clean: items that do not contain
the organisms; protect these areas
from contamination
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Classifications of
Precautions
Standard precautions
Airborne precautions
Droplet precautions
Contact precautions
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Protective or
Reverse Isolation
Method used to protect certain
patients from organisms present in
the environment
Used mainly for
immunocompromised patients
Precautions vary depending on
patient’s condition
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Summary
Exact procedures for maintaining
transmission-based isolation will vary from
one facility to another
Variations caused by different factors
Know and follow procedures at your facility
Basic principles remain the same in any
facility and are directed toward preventing
the spread of disease
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