Transcript Slide 1

Perio Protect Method™
Scanning Electron Microscope
Bacterial Count Results
D.C. Keller, Perio Protect LLC, St. Louis, MO, USA; J.W. Costerton, USC School of
Dentistry, Los Angeles, CA, USA; C. Schaudinn, USC School of Dentistry, Los Angeles,
CA, USA; P. Sedghizadeh, USC School of Dentistry, Los Angeles, CA, USA; G.S.
Keller, Keller Professional Group P.C., St. Louis, MO, USA
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Perio Protect Method™
Localized Medication Delivery System
The Perio Protect Method™ uses an appropriately formed,
customized dental tray fabricated in a licensed dental lab to
place prescribed solutions into periodontal pockets.
Flexible Polycarbonate Carriers
Flexible polycarbonate
carriers were inserted into
periodontal pockets and
worn in vivo for two day
intervals during treatment
with the Perio Protect
Method™.
The pictures in this
presentation show
beginning bacterial biofilm
and subsequent SEM
pictures taken after 2
days, 7 days, 12 days,
and 17 days of treatment.
© Perio Protect
Perio Protect Method™
Localized Medication Delivery System
19 mb, before Perio Protect® Treatment
© Perio Protect
19 mb, before Perio Protect® Treatment
The magnified biofilm has
formed on the carrier and
consists of those bacteria
present in the 6mm
periodontal pocket. These
can be virulent strains, such
as Porphyromonas gingivalis,
Actinobacillus
actinomycetemcomitans,
Treponema, etc.
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19 mb, 2 days after Perio Protect® Treatment
© Perio Protect
19 mb, 2 days after Perio Protect® Treatment
© Perio Protect
19 mb, 2 days after Perio Protect® Treatment
This slide demonstrates a
much modified oral
bacterial environment after
2 days of treatment. The
colonization of fusiform-like
bacteria and long rods has
been affected by the
patient delivered
medications and some of
the cocci-like bacteria also
demonstrate modifications
in form.
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19 mb 7 days after Perio Protect® Treatment
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19 mb, 7 days after Perio Protect® Treatment
These results after using
the medications for 7 days
demonstrate a dramatic
change in the bacterial
colonization. The bacterial
population is decreased in
this study by 98% and there
are no rods observed in
these scans. The
remaining biofilm bacteria
demonstrate significant
modifications.
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19 mb, 12 days after Perio Protect® Treatment
© Perio Protect
19 mb, 12 days after Perio Protect® Treatment
The periodontal pocket is
significantly changed by 12
days. There are almost no
bacteria observed and the
pellicle is observed to be
incompletely formed on the
carrier through the action of
the medications where
hyaluronic acid is cleaved
and histadine is converted
to alanine and asparagine is
converted to aspartate and
the protein chains are
disrupted.
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19 mb, 17 days after Perio Protect® Treatment
© Perio Protect
19 mb, 17 days after Perio Protect® Treatment
99.98% of the bacteria are
missing from the carriers at
17 days. Those having the
form of Actinomyces-type of
bacteria and a few coccilike bacteria are all that can
be found and we know
these from the normal
aerobic microflora of
healthy gums.
© Perio Protect
Perio Protect Method™ effect on subgingival biofilms
(graph) tooth 19 mesial buccal
4
5
3
3
Probing Changes after 12 days
4
4
2
3
2
1
3
2
100%
100%
~2x10E6 ~2x10E6
106
Fusiform-like
Selenomonas-like
Cocci-like
bacteria cells
short rods
105
0.25%
~4x10E3
~5x10E3
104
103
0.02%
~4x10E2
0
Actinomyces-like
0.2%
2
7
12
days
17
© Perio Protect
2x10E7
14mb
1%
2x10E5
1%
2x10E5
short rods
0.05%
1x10E4
0%
0x10E0
~3x10E7
carrier lost
Selenomonas-like
14db
Cocci-like
100%
Actinomyces-like
carrier lost
Fusiform-like
2%
~6x10E5
0.002%
~7x10E2
100%
~2x10E6 ~2x10E6
19mb
0.2%
© Perio Protect
0
2
~4x10E3
0.25%
~5x10E3
7
12
0.02%
~4x10E2
days
17
Cell analysis before treatment with the Perio Protect Method™ determined the
pocket ecosystem consisted of large numbers of fusiform-like bacteria, cocci,
short rods and in one pocket, treponema. After two days of using the Perio
Tray™ for minutes each day according to the Perio Protect™ protocol most of
the fusiform bacteria and treponema had disappeared. After 17 days there
were less than 0.02% (99.98% Kill) of the periopathogenic bacteria and what
remained were some cocci and pleomorphic rods that were visible next to a
large number of eukaryotic cells.
After 17 days the few remaining bacteria, cocci- and actinomyces-like,
are known as normal aerobic microflora of healthy gums.
End of Presentation
© Perio Protect