Transcript Nano + Bio: A Metrology Challenge

Nano + Bio:
A Metrology Challenge
NAWI
May 2008
Deb Newberry
Newberry Technologies
Dakota County Technical College
[email protected]
New technology could combine
detection and treatment
Immediate sensing as a tumor is removed!
© Deb Newberry
2002,2003,2004,2005,2
Sandia National Laboratories
Challenge and Opportunity
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We always want and need to measure what we created
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1) to find out if we actually made what we thought
2) to determine the quality of what we made
3) to understand and measure stability of the process
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Dealing at the nanoscale is tough
Dealing with biological systems is tough
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Combining nanoscale and biology increases the difficulty of the problem
perhaps exponentially
Combination Poses the Challenge
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Nanoscale
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Method
Tools
Accuracy of what we measure
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Consistency
Statistics
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What does it mean to “measure” an atom????
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Tolerable error bars
Accept/Non accept conditions
Unknown consequences
Consider the Target: Complicated, Integrated Biological System
Combination Poses the Challenge
Statistics
Repeatability
Efficacy over Time
Time factor
-Storage
-Application
Accuracy
Nanotechnology
Aspects
Biological
Aspects
Purity
Verification
Sample Size
Equipment
Methodology
Regulation
Regulatory
Required
Possible
Nanotechnology
Aspects
Fabrication
Application
- Verification
- Purity
- Continuity
- Diagnostic
- Preventative
- Topical
- In vivo
Metrology
- Methods
Assessment Environment
- Tools
- Statistics
- Timing/frequency
Societal
Effects
- Intended
- Adverse
Biological
Aspects
NanoToday, June 2007
Chang-Cheng Youa, Apiwat Chompoosora and Vincent M. Rotelloa,
aDepartment
of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, MA 01003
, USA
Au core nanoparticle
Coated with biological material
Imbed in cancer cell
Via extra body energy
Heat kills cancer
Integrity of of coating
-Continuity
-Purity
Patient matching
Au nanoparticle
60% detection material
40% toxic material
Measurement of a beaker full may be pretty straight forward
Measuring quality of individual NPs is not possible
60/40 ratio
Quality of coating
Amount used for treatment
Biological Aspects
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If we measure it at day 1 will it work at day 200?
Storage environment
Application environ chemo/radiation
Application method
Human target
Tailor-ability history and integrity
Regulatory
Required
Possible
Nanotechnology
Aspects
Fabrication
Application
- Verification
- Purity
- Continuity
Metrology
Education
- Diagnostic
- Preventative
- Topical
- In vivo
- Methods
Assessment
Environment Effects
Communication
- Tools
- Intended
- Statistics Common Methodologies
- Adverse
- Timing/frequency
Collaboration
Societal
Biological
Aspects
Student Assignment
Manf, QA, Rel. Class
Topic: FMEA
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DCTC Nanoscience Technology AAS Degree
72 credit program (Over half are nano - specific courses)
Multi-disciplinary
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Customer (Employer) Needs
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Nanoelectronics (IBM, Seagate, Honeywell, Goodrich, Cypress et.al.)
Nanobiotech (Medtronic, St. Jude, SciMed, Surmodics et. al.)
Nanomaterials (3M, HB Fuller, Entegris, Hysitron et.al.)
Equipment knowledge (AFM, SEM, XRay, TEM, nanomechanical)
Concept understanding (Forces, scale, SA/Vol, QM, cell, Dogma, bonding etc.)
Team operation (Data analysis and presentation, oral and written skills)
Third Semester Course
2 Examples to select for FMEA
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Au nanoparticle
CNT/Stem Cell combo – used as scaffold to rebuild neural connections
Hardware
Item
Failure Modes
Causes of Failure
Failure Effects
Severity
Probability
of
Occurrence
Criticality
Comments
CNT
Doesn’t absorb
Lamanin
CNT is the wrong
shape or size
Stem cells don’t attach to
the CNT’s and go to
healthy part of brain
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With proper
sorting of
CNT’s this
should be
preventable
Short wires the brain
CNT’s in the brain
cause parts of the
brain to function in
correctly
Serious change in patients
brain function
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Research how
the presence
of CNT’s
affect the
function of
neurons
CNT’s attacked by
immune system
No or incorrect bio
coating
Clots in the brain,
possible hemmoraraging
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Research the
immune
response to
CNT’s
Don’t attach to CNT’s
Lack of lamanin in
CNT
Stem cells regenerate in
healthy part of brain
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Learn to
control the
stem cells
attachment to
the CNT
Don’t differentiate into
neurons
Cell wasn’t in proper
environment
Treatment was a waste,
CNT’s left in brain
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Further
research on
stem cells to
insure how
they react and
differentiate
Stem Cells
Unanswered Questions
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The usual
– Method
– Equipment
– Statistics – error bars
Patient defined
– Coating – biological – genome dependent?
– Timing
– Purity
– Efficacy
Regulatory
– Simulation vs test
Societal
– Expectations
– Demands
Thank you!