Transcript The Effect of Zinc Status on Proinflammatory Response

THE EFFECT OF ZINC STATUS ON
PROINFLAMMATORY RESPONSE
IMPLICATIONS FOR AGE-RELATED
CHRONIC INFLAMMATION
Nicole Rinaldia, Carmen Wong, PhDb, Emily Ho, PhDb,c
aDepartment
of BioResource Research
bSchool of Biological and Population Health Sciences
cLinus Pauling Institute,
Oregon State University, Corvallis, OR 97331, USA
BACKGROUND
 Zinc is an essential micronutrient
 Important role in immune system
health
 Zinc deficiency causes chronic lowgrade inflammation
 Chronic inflammation promotes development of
degenerative disease
 Heart disease, diabetes, autoimmune disease, cancer
BACKGROUND
 Aging is strongly associated with chronic

inflammation
Zinc status declines with age
 12% of total US population is zinc deficient,
however 40% of elderly Americans are zinc
deficient
 Due to both decreased absorption of zinc and
consumption of zinc in the elderly
RESEARCH QUESTIONS
 What level of zinc deficiency causes immune
dysregulation?
 Can zinc supplementation improve immune
function?
HYPOTHESIS
 Zinc deficiency will increase the
intensity of the proinflammatory
response compared to that elicited
by zinc adequate conditions
 Zinc supplementation will decrease
the intensity of the proinflammatory
response compared to that elicited
by zinc adequate conditions
METHODS
 THP-1 human leukemic monocyte cell culture model
 Monocytes are a form of white blood cell involved in

proinflammatory response
Cells are cultured in media that is either zinc deficient
(0 μM zinc), marginally zinc deficient (1 μM zinc), zinc
adequate (4 μM zinc), or zinc supplemented (40 μM
zinc)
METHODS
Day 25 ZA
Day 25 ZD
 Monocytes undergo measurable processes during
induction of proinflammatory response
 Increased ICAM1 expression, increased production of
proinflammatory cytokines, increased generation of
reactive oxygen species (ROS)
RESULTS
Expression of Cell Activation Marker ICAM1
Production of Proinflammatory
Cytokines IL1β & IL6
ZA= zinc
adequate
MZD= marginal
zinc deficient
Production of Reactive Oxygen Species (ROS)
ZD= zinc
deficient
ZS= zinc
supplemented
CONCLUSIONS
 Effect of zinc supplementation on
intensity of proinflammatory
response is unclear at this time
 Zinc deficiency and marginal zinc
deficiency increase intensity of
proinflammatory response
 Increased production of cell

activation markers, proinflammatory
cytokines, and ROS
Zinc deficiency and marginal
zinc deficiency may contribute
to chronic inflammation
experienced by the elderly
ACKNOWLEDGEMENTS
 LIFE Scholars Summer Research Program
 Dr. Emily Ho – Mentor
 Endowed Director, Professor, Principal Investigator
 OSU College of Public Health and Human Sciences
 Dr. Carmen Wong – Mentor
 Research Associate, Nutrition & Exercise Sciences
 OSU College of Public Health and Human Sciences
 Wanda Crannell – Advisor
 Advisor / Instructor
 OSU College of Agricultural Sciences
RESULTS