Transcript Lab 5 Microscopy PPS

By Jane
Horlings
Microscopy
Microscopy
Robert Hooke, circa 1700s
Electron Microscope, late 1900s
Light Microscopes
Early light microscope (UL), drawing by Hooke (LL)
Light Microscopes
Dissecting (stereo) microscope (L), compound microscope (R)
Light Microscopy; Plant Cells
Onion root tip, cell division (L), shoot tip (R)
Light Microscopy, Phase Contrast
Single celled Amoeba dividing (L), green alga Micrasterias (R)
Light Microscopy
Fluorescence
Confocal
Phase contrast
Electron Microscopes
Limitations of Electron Microscopy
• Works in a vacuum
• Specimens are dead,
chemically preserved; no
life processes can be
seen
• No color (colorized by
artist on computer)
Electron Microscopes
Transmission Electron Microscope
(TEM)
Scanning Electron Microscope
(SEM)
Scanning Electron Microscopy
TEM of cilia
SEM of cilia
Bacterium
RBCs in clot
SEM
SEM
Mite (UL)
Gecko toes (LL)
Shark skin (LR)
Trypanosome
Red blood cells
Red blood cells and
Trypanosoma (L), Giardia (R)
SEM
Transmission Electron Microscopy
TEM of cilia
SEM of cilia
TEM
Viruses (L), animal cell (R)
TEM
Animal cell (L), muscle tissue (R)
Light Microscopy
• Based on light
• Specimens can be
alive; life processes
can be seen
• Color; dyes may be
used
Parts of the Microscope
• Ocular lenses
• Objective lenses
• How to compute
the magnification
Use of the Microscope
• Place slide in center
• Adjust light, lenses, barrel
– Put on low magnification!
– Move stage all the way up
and then back down half a
turn!
Use of the Microscope
• Look and readjust focus
• Move to higher
magnification if needed
Use of the Microscope
• Importance of focusing
with the fine adjustment!
• Oil immersion lens
• How to adjust the light
End