Transcript Cry3A transgenic, bark beetle resistant spruce

NAZV project
QH71290
2007-2011
Cry3A transgenic, bark
beetle resistant spruce
Spruce bark beetle
(Ips typographus)
Bark beetle galleries
Bacillus thuringiensis
crystal protein (Cry, δ-endoTOXin)
Agrobacterium interaction with the plant cell
Major problems:
Spruce is difficult to transform
No known Bt toxin is toxic enough to bark
beetle
Spruce callus cultures
Somatic embryogenesis
induction
conversion
cultivation
Modified Cry3A genes
Cry3A orig
Synt TOX-1 (602 aa)-codons Picea optimized
Ndel/sTOX-1
Cdel/sTOX-1 (602 aa)
Cdel/Ndel/sTOX-1
GUS
Refined modification of
domain II
Expression vector
PAGE analysis of modified Cry3A
expression
WESTERN detection of modified
Cry3A proteins
Toxicity tests on live beetles
Konec
presentace