Transcript Lab #5 Rate of Cellular Respiration

AP Bio Lab #5
Cellular Respiration
The process by which plant tissue expands in
tight places involves water uptake through
osmosis and imbibition, and an overall increase
in size by cell division. In the case of an oak or
pine tree splitting open a granite boulder, the
amazing process starts with a seed that
becomes lodged in a tiny crevice. Like a living,
expanding wedge driven deeper and deeper into
the crack, the boulder is literally forced apart by
tremendous pressures.
Seed
Structure
A seed consists of three
parts:
1) a Dormant
Embryo,
2) a Storage
Tissue,
3) a Seed Coat.
Seed
Structure
A seed consists of three
parts:
1) a Dormant
Embryo,
2) a Storage
Tissue,
3) a Seed Coat.
Seed
Structure
A seed consists of three
parts:
1) a Dormant
Embryo,
2) a Storage
Tissue,
3) a Seed Coat.
No seed germinates, unless it
absorbs water (imbibition)
 Water
hydrates an
enzyme
after water absorption
before water absorption
abscisic acid, plant hormone, keeps
the embryo dormant (inhibits
seed germination)
 Water hydrates an
enzyme
 This enzyme
breaks down
(metabolizes)
abscisic acid
Germination & subsequent
growth require A GREAT DEAL
OF ENERGY
Young plants get this ENERGY by
converting the energy of food
molecules (starch in endosperm or
cotyledon) to ATP during CELLULAR
RESPIRATION
So… Lab #5 Cellular
Respiration
O2 enters the
mitochondria
ATP is generated
(made)
CO2 exits the
mitochondria
When you study the equation for cellular
respiration, you will see that there are at least three
ways to measure RATE:
1.
2.
3.
Measure the amount of glucose consumed.
Measure the amount of oxygen consumed.
Measure the amount of carbon dioxide produced.
In this experiment, we are going to
measure the amount of oxygen
consumed.
You will use a RESPIROMETER
CO2
Determine the
rate of O2
consumption
Y = change in volume
X = change in time
Y = change in volume
.24mL - .06mL = .16
Y = .16 mL
X = 10 min
Y
X
= .016 ml / min