Transcript Cultivation of plant viruses Protoplast and callus method

Cultivation
To propagate the viral particle
in its specific host i.e.
Increase viral concentration
through support viral
replication.
Cultivation
Animal
Bacteria
Plant
Insect
Methods of animal virus
cultivation
1. Organized tissue.
2. Cell culture.
3. Laboratory animals.
Chick embryo technique
Methods of animal virus
cultivation
1. Organized tissue.
2. Cell culture.
3. Laboratory animals.
Cell culture
1.
2.
3.
4.
5.
6.
7.
8.
Removal.
Homogenization.
Washing.
Trypsinization.
Washing.
Growing.
Inoculation.
Incubation.
Methods of animal virus
cultivation
1. Organized tissue.
2. Cell culture.
3. Laboratory animals.
Intracerebrally
Intraperitoneally
Intramuscularly
Intravenously
Intranasally
Types of cell culture
Primary
Suspension
Diploid
Permanent
Primary cell culture
A culture derived directly from a tissue
Diploid cell culture
Subsequent cultures derived from primary cell culture.
Suspension cell culture
A culture in it the dispersed cells are not allowed to settle down, but
they are kept floating by continuous shaking of the flasks.
Permanent cell line
A culture derived from single separated cell i.e. diploid culture it can be
propagated indefinitely.
Cultivation of plant viruses
• Protoplast and callus method.
• Mechanical method.
• Biological method.
Cultivation of plant viruses
• Protoplast and callus method.
• Mechanical method.
• Biological method.
Inoculation
Incubation
Inoculation
Incubation
Cultivation of plant viruses
• Protoplast and callus method.
• Mechanical method.
• Biological method.
Cultivation of plant viruses
• Protoplast and callus method.
• Mechanical method.
• Biological method.
Incubation
Cultivation of bacterial viruses
1. On broth media
2. On solid medium
Virus + semisolid agar
Bacteria + solid agar
Cultivation of Insect viruses
Criteria of purity
Chemical purity
Physical purity
Biological purity