Transcript Microscope

Introduction to the
compound Microscope
Types of Microscopes
Onion cells (100x)
Cheek cells (400x)
Compound Microscope
Two spotted
spider mite (20x)
Stereoscope or
dissecting scope
Types of Microscopes
TEM - Transmission
Electron Microscope
Types of Microscopes
SEM - Scanning
Electron Microscope
Revolving nosepiece
Eye piece
Objectives
Stage Clips
Stage
Diaphragm
Body Tube
Coarse Adjustment
Fine Adjustment
Arm
Mirror (light source)
Base
Rules for using the microscope
• Always carry with 2 hands. Place one hand on the
arm and one hand on the base.
• Make sure the microscope is on a flat surface and
away from the edge of the table.
• Always Look to the side when changing objectives
and rotate carefully.
• Always use low (scanning) power first.
• Always store the microscope covered and with the
lowest powered objective in place.
• Only use lens paper for cleaning
Microscope Basics:
Determining Total Magnification
10 x
• Multiply the magnification
of the ocular lens
(eyepiece) by the
magnification of the
objective lens.
Total magnification = 10 x 4 = 40
We represent this magnification as 40X
4x
Microscope Basics:
Field of view
• Field of view is what you see when you look into
a microscope.
As you increase
magnification your field of
view becomes smaller
Microscope Basics: Depth of focus.
• Refers to how much of the specimen you can
focus clearly on at one time in your field of view.
• As you increase magnification your depth of
focus becomes narrower and less of your sample
can be focused at one time.