Transcript Microscope Parts and Function

Microscopes
Microscope Parts Quiz on Friday!!
Will need to label parts on diagram
and complete matching/mult. choice
on the function of the parts.
Microscopes
This week’s objectives:
•To name the parts of the microscope
and describe their functions
•To describe how to properly use a
compound microscope.
•To compare a compound light
microscope to a dissecting
microscope and electron
microscopes.
Label the parts on your
microscope picture.
Eyepiece
magnification
= 10X
Label the parts on your
microscope picture.
Eyepiece
magnification = 10X
Arm-Supportproper place to pick
up/carry
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage – slide is
placed here for
viewing
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust –
general focus. Use
ONLY on Scanning
power.
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust –
general focus
Fine Focus – use
for high power
focus
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust –
general focus
Fine Focus – high
power focus
Base – supportplace other hand
here when
carrying.
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Light source
Has a dimmer
switch to adjust
the amount of
light
Coarse adjust –
general focus
Fine Focus – high
power focus
Base - support
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Diaphragm – also adjusts
amount of light-works like
the pupil of your eye.
Light source
Coarse adjust –
general focus
Fine Focus – high
power focus
Base - support
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Stage clips – holds slide
Diaphragm – adjusts
amount of light
Light source
Coarse adjust –
general focus
Fine Focus – high
power focus
Base - support
Label the parts on your
microscope picture.
Objective Lenses
Eyepiece/ocular lens
magnification = 10X
Arm-Support
Scanning = 4X (shortest)
Low Power = 10X (med. length
Stage - support
High
40X slide
(longest)
Stagepower
clips –=holds
Coarse adjust –
general focus
Fine Focus – high
power focus
Diaphragm – adjusts
amount of light
Light source
Base - support
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Revolving nosepiece
Objective Lenses
Scanning = 4X (shortest)
Arm-Support
Low Power = 10X (med)
High power = 40X (longest)
Stage clips – holds slide
Diaphragm – adjusts
amount of light
Light source
Stage - support
Coarse adjust –
general focus
Fine Focus – high
power focus
Base - support
Label the parts on your
microscope picture.
Eyepiece/ocular lens
magnification = 10X
Tube
Revolving nosepiece
Objective Lenses
Scanning = 4X (shortest)
Arm-Support
Low Power = 10X (med)
High power = 40X (longest)
Stage clips – holds slide
Diaphragm – adjusts
amount of light
Light source
Stage - support
Coarse adjust –
general focus
Fine Focus – high
power focus
Base - support
Magnification of a Compound Microscope
•Because you are looking through multiple lenses the lenses have a
“compounding” effect.
•The eyepiece always magnifies 10X
Eyepiece
Scanning lens (red)
Low Power (yellow)
High Power (blue)
10X
10X
10X
Objective Lens
Magnification
Magnification of a Compound Microscope
•Each of the objective lenses have their own magnification
Eyepiece
Scanning lens (red)
Low Power (yellow)
High Power (blue)
Objective Lens
10X
4X
10X
10X
40X
10X
Magnification
Magnification of a Compound Microscope
•You then multiply the eyepiece with the objective lens to
determine the total magnification
Eyepiece
Scanning lens (red)
Low Power (yellow)
High Power (blue)
Objective Lens
Magnification
10X
4X
40X
10X
10X
100X
10X
40X
400X
General Procedures
•Always carry the microscope with one
hand on the Arm and one hand on the
Base.
•Wear your glasses, the microscope will
focus to your eyesight!
• Keep both eyes open, your brain will learn
to ignore the other eye.
• Never use the coarse adjustment after
you turn past scanning (4X) power.
Focusing a Specimen

Always start on scanning power.
 Bring the stage all the way up and focus the
specimen using the course adjust.
 Re-center so what you want to focus is at the
pointer!
 Turn to high power only after re-centering
 Use the FINE FOCUS ONLY for low and highest
power. NEVER use the course adjustment after
you turn past scanning power.
Making good drawings
1.
Don’t even think of starting your drawing unless you have a pencil
(colored are even better) drawings in pen are unacceptable. This is
for two reasons:
(a) You can erase pencil!
(b) You can shade in areas more easily in pencil
2.
Take time to add detail to your drawing. Use your pencil to shade and
give your drawing some detail and depth. Use colored pencil when
appropriate to add color.
3.
In the upper left hand corner of each circle include the specimen
name as written on the slide label. In the upper right hand corner,
include the magnification (100x or 430x).
4.
Label Everything you identify!
Cheek cell
100X
Cell membrane
nucleus
Making a Wet Mount Slide
1. Gather a very thin slice/piece of whatever your
specimen is. All samples should be paper thickness or
thinner!
2. Place ONE drop of water directly over the specimen.
The drop should be about ½ the size of a dime.
3. Place the cover slip at a 45 degree angle with one
edge touching the water drop, and let the cover slip drop
onto the drop of water.
4. Do not push down on the cover slip.
5. Try to avoid bubbles!
Microscopes
Microscope Parts
Label with structure
and function
Magnification of Lenses
Eyep
iece
Scanning lens
(red)
Low Power
(yellow)
High Power
(blue)
10X
10X
10X
Objectiv
e Lens
Magnificat
ion