Transcript Microscope Basics

T. Trimpe 2005
http://sciencespot.net/
Base
-Single piece of
metal
-Support here when
moving
Color RED
Arm
-Hold here
when moving
Color Dark
GREEN
Stage
-Supports
Specimen
-Hole allows light
to pass through
 Color BLUE
Diaphragm
-controls
amount of light
-ranges 1-5
Color
LIGHT
GREEN
Stage Clips
-secures specimen/slide
 Color GREY
Bulb/Mirror
-light source
-NEVER use the
sunlight it will
hurt your eyes!!
Color
YELLOW
Scanning Objective Lens
-shortest lens
-4x magnification
Color BLACK
Low Power Objective Lens
-10x
magnification
Color
PURPLE
High Power Objective Lens
-40x
magnification
Color
ORANGE
STRIPES
Body Tube
-allows light
from objective
to pass upward
Color
BROWN
Eye Piece/Ocular lens
-Site of
observation
-10x magnification
Color ORANGE
Nose Piece
-Allows you to
switch objective
lenses
Color BLUE and
GREEN stripes
Coarse Adjustment Knob
-used to focus
specimen
-DO NOT use on
high power
objective lens
Color PINK
Fine Adjustment Knob
-Used for minute
focusing
-Used after coarse
adjustment knob
Color Tan
• Total magnification = (ocular lens power) x (objective lens
power)
• Example: (10x)oculear lens x (40x)high power lens = 400x
total magnification
• Magnification: The ability to increase the size of an image
X
Ocular Lens
= 400x total
magnification
Objective Lens
Light Pathway: Light Source→Specimen→Objective Lens→Body
Tube→Eye Piece
Resolution
• making the image clear
• ability to determine between two objects or
points
• can be adjusted with the fine/course knob or the
diaphragm (to allow more light in)
Comparing Powers of Magnification
We can see better details with higher the
powers of magnification, but we cannot see
as much of the image.
Which of these images
would be viewed at a
higher power of
magnification?
Use of a Scope

Carry the microscope with 2 hands (arm & base)

Use closest electrical outlet without going through a walkway

Looking at a slide:

Use ONLY ONE slide at a time

ALWAYS start at LOW power first(10x)

ALWAYS start with the stage lowered completely

Make sure the objective clicks into position

The course focus knob is ONLY used on the lowest
power lens

The fine focus knob is used on all lenses to bring the
image into focus
Putting the Microscope Away

Lower the stage completely and click the lowest
objective into place

Return slide to tray

Wind up the scope power cord
Agenda
1. Finish Microscope Handout
•
Glue in notebook when finished
•
Due tomorrow
2. Study Guide
•
Answer on separate piece of paper
•
Due Friday
3. Extra Credit Flashcards
•
Make 20
•
Due Friday
Types of Microscopes
1. Compound Light
2. Electron microscope:
Microscope: Shines
Uses electrons instead
light through a
of light to visualize the
specimen and uses 2
specimen, electrons
lenses for magnification bounce off or pass
through and a computer

Magnification – increase
interprets a picture
in an objects apparent
size


Resolution – how clear
the image appears once
magnified
Max clear magnification
~2000x
Always black and white,
specimen must be dead to
examine
Scanning EM – magnifies up
to 100,000x
Transmission EM – magnifies
up to 200,000x
Microscope Images
Compound Light Microscope - Protozoa
Scanning Electron Microscope (SEM
Butterfly Tongue
Transmission Electron Microscope - Bacteria