Transcript Diapositive 1

Kickoff meeting of MarPlan
September 23rd
Napoli
Observatoire de Villefranche
Laboratoire d’Océanographie de Villefranche
Maria Luiza Pedrotti
Rodolphe Lemée
Alina Tunin
John Dolan
Illustrations from website: www.obs-vlfr.fr
Observatoire Océanologique
de Villefranche-sur-Mer
French coast of NW Mediterranean Sea
Zoological Station
Bay of Villefranche-sur-Mer
Observatoire
- 130 permanent researchers or
technicians
- Surface : 7 000 m2
- One restaurant (for lunch) and
accommodation
Sagitta
Vellele
Bay of Villefranche-sur-Mer
Daily plankton samples
Observatoire Océanologique de
Villefranche-sur-Mer
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University of Paris VI (with Banyuls and Roscoff)
and CNRS: National Center of Scientific Research
Center of Research, Monitoring and Teaching
Teaching: Masters with marine ecology (pelagic
and benthic systems), plankton diversity,
biogeochemistry, numerical ecology, geology.
Monitoring
Pt B
SOMLIT station
DYFAMED - JGOFS station
28 miles offshore
Research
Multidisciplinary
research center with 3 laboratories:
Geosciences
Research topics:
- History, mechanical
properties and
deformation of the
lithosphere,
- Natural hazard
assessment
Azur
Developmental Biology
Reaserch topics:
- Cell cycle control
- Embryonic development of marine
invertebrate
Expertise:
- Light microscope imaging of calcium,
cytoskeleton and organelles in live cells
- Subcellular visualisation and localisation
techniques (immunolocalisation at light and
electron microscopy, fast-freeze/deep etch)
- Biochemistry
- Molecular Biology (cloning, expression
studies, in situ hybridisation)
Laboratoire d’Océanographie de
Villefranche (LOV)
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31 researchers, 21 engineers/technicians, 10
post-Doctorals positions and 18 Ph.D
5 research groups specialized in pelagic systems
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Marine optic and remote sensing
Geochemistry
Dynamic of plankton
Biological and physiological answers to stress
Marine microbial ecology (involved in marPLAN)
Marine optics and remote sensing
It is an interdisciplinary group
studying the interaction of solar
of solar energy with the ocean.
BOUSSOLE: New platform dedicated to optical
Observations in the surface layer of the ocean
Geochemistry
Effect
of nutrients and metals on pelagic systems
Evolution of primary production and pigment diversity
in NW Mediterranean Sea (monitoring at DYFAMED site)
Dynamic of plankton
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Plankton diversity and structuration of pelagic food web in
relation to trophic and environment.
Role of key species in energy transfer
Phytoplankton ecophysiology and modeling
Biological and physiological answers
to environmental stress
Spatio-temporal
variability and adaptative
strategies of microzooplankton and necton
Quantification of zooplankton impact on
biogeochemical cycles
Marine microbial ecology
7 researchers, 2 engineers/technicians, 3 post-Doctoral
researchers, 4 Ph.D students and 6 or 7 master students/years
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majors topics of the group:
 Factors regulating activities and structure of
microbial populations (external forcing, top-down,
bottom-up controls)
 Functional and taxonomic diversity in microbial
populations: variation in time-space scale
 Roles of microbes in biogeochemicals processes
(dynamics of DOM, TEP and CO2)
Marine microbial ecology
Permanent researchers and expertises
Involved in MARPLAN
-John Dolan, group leader:
Diversity and ecology of ciliates
-Rodolphe Lemée, assistant professor:
Diversity and ecology of microphytoplankton
-Maria Luiza Pedrotti, research scientist: Bacterioplankton
diversity and role of TEP in the biogeochemical cycles
John
-Alina Tunin (Ph.D -University of Paris VI, Ibanez and Lemée):
Short, middle and long-term changes in Ceratium diversity
Rodolphe
Others
-Jean-Pierre Gattuso, senior research scientist:
Biogeochemical processes, bacterioplankton respiration
-Emilio Maranon, research scientist:
Maria Luiza
Ecophysiology of phytoplankton
-Fereidoun Rassoulzadegan, senior research scientist:
Microbial ecology, effect of nutrients on marines microbes
-Markus Weinbauer, research scientist:
Alina
Diversity of marine bacteria and viruses
Marine microbial ecology
Equipment and techniques:
Epifluoresence microscopes with image analysis
Coulter counter
Flow cytometer (FacsCalibur)
TOC analyzer (Shimadzu)
Tangential flow filtration system (Millipore)
Titrators for O2 and total alkalinity
determinations
High precision pH meters
Incubators, Spectrometer
System for pCO2 control of algal cultures
Respirometers with Peltier-regulated
temperature
FISH, CARD-FISH
Pulse gel electrophoresis, PCR, DGGE
Specific needs from the collaboration within MarPlan
Long-term changes in Ceratium (dinoflagellate)
diversity in Ligurian and Tyrrhenian seas
Alina
Tunin and Rodolphe Lemée
Needs:
Old
and recent net samples of microphytoplankton
Literatures from cruises, reports and old papers
on distribution and annual cycle of Ceratium
Relationship between morphological and taxonomic
diversity in tintinnid cilates
Quic kT ime™ et un
déc ompres seur T IFF (non compress é)
s ont requis pour vis ionner cette image.
John
Dolan
Needs:
Samples
from diverse sites,
expertise in morphometrics
Phylogenetic relations at specific and subspecific
levels in genus Ceratium
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Rodolphe Lemée
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Needs:
« Single cell PCR technique » :Research collaboration
during 2005 and 2006 with phytoplankton group of Roscoff
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Several Ceratium tripos
Same specie ?
Illustration from Sournia
Bacterial diversity and dynamic of particles
(TEP transparent exopolimeric particles)
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Maria Luiza Pedrotti
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Needs:
Exchanging samples and calibration protocols for FISH
and CARD-FISH
What bacterial group colonize particles ?
Bacteria
TEP
Superposition