Transcript Lecture 1 - Hormone Action

Mechanisms of hormone actions
Endocrine 412
Objectives
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Defining hormones and their classifications.
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Listing the general characteristics of hormones.
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Identifying factors determining the response of target cells to hormones.
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Describing the different mechanisms of action of hormones.
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Listing hormones that bind to cell surface receptors.
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Listing hormones that bind to intracellular receptors.
General characteristics of hormones
Hormones :
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are molecules that are produced by special cells (not by all cells of the body)
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are secreted directly to blood (with no ducts)
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are available in very low concentrations in blood
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are transported in blood to work in distant sites
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have systemic actions
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have specific receptors (either intra- or extra-cellular ) in target tissues
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have different special mechanisms of actions
Receptors of hormones
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Hormones are present in very low concentrations in extracellular fluids (e.g. blood)
In order to initiate their biologic effects, target cells must recognize hormones by
binding to specific receptors
A target cell is defined by its ability to bind selectively a given hormone via a receptor
Receptors have at least 2 DOMAINS:
1- Recognition domain: binds to the hormone
2- Coupling domain: couples hormone recognition to some intracellular function by
generating a signal
Generally, COUPLING occurs by two ways:
1- Polypeptide hormones, protein hormones & catecholamines binding of hormones to
plasma membrane receptors (extracellular receptors).
2- Steroid & thyroid hormones interact with intracellular receptors, form complexes that
generates a signal.
The concentration of hormone as seen by target cells is
determined by three factors:
1-Rate of production:
Synthesis and secretion of hormones are the most highly regulated aspect of
endocrine control. Such control is mediated by positive and negative feedback circuits
The concentration of hormone as seen by target cells is
determined by three factors: (cont.)
2-Rate of delivery:
An example of this effect is blood flow to a target organ or group of target cells –
high blood flow delivers more hormone than low blood flow.
3-Rate of degradation and elimination:
Hormones, like all biomolecules, have characteristic rates of decay, and are
metabolized and excreted from the body through several routes.
Hormones are cleared by:
1- Metabolic destruction by tissues
2- Binding with tissues
3- Excretion by the liver into bile
4- Excretion by the kidney into urine
5- Clearance of protein bound hormones is slower than clearance of peptide hormones
Classifications of hormones
Hormones are classified according to:
1- Chemical composition (protein or others)
2- Solubility (hydrophilic or lipophilic)
3- Location of receptors (intra- or extra-cellular)
4- Nature of signal used to mediate hormone action within cells
According to their classifications, hormones are classified into
Group I & Group II (according to mechanism of action)
Classification of hormones according to mechanism of action:
Group I
Hormones that bind to intracellular receptors (cytosolic or nucleus):
1- Solubility: lipophilic (to pass cell membrane which is mainly lipid)
2- Chemical composition:
Derived from cholesterol (except: thyroid hormones & retinoic acid)
3- Transport in blood:
Associate with transport proteins (to tackle solubility problem)
Long plasma half-life
4-Mechanism of action:
1- Free hormone traverses plasma membrane of cells & binds to receptor in cytosol or nucleus
of target cells.
2- Hormone-receptor complex binds to specific region in DNA of gene (hormone-response
element, HRE)
3- Binding causes activation or inactivation of specific gene(s) transcription (production of
mRNA)
4- Accordingly, amount of protein synthesis (production) is changed (protein may be enzymes
of metabolic react.)
5- Accordingly, certain metabolic processes are affected.
Example of Group I hormones:
Steroid hormones via cytoplasmic receptors
Example of Group I hormones:
Retinoic acid (active product of vitamin A) via nuclear receptors
List of Group I hormones
• Thyroid hormones
• Steroid hormones:
Glucocorticoids (e.g. cortisol hormone)
Mineralocorticoids (e.g. aldesterone)
Androgens (e.g. testosterone hormone)
Estrogens
Progestins (e.g. progesterone hormone)
• Retinoic acid (active metabolite of vitamin A)
• Calcitriol (1, 25 DHCC, active metabolite of vitamin D)
Classification of hormones according to mechanism of action:
Group II
Hormones that bind to plasma membrane (extra-cellular) receptors:
1- Solubility: hydrophilic
2- Chemical composition:
Polypeptides, proteins, glycoproteins & catecholamines
3- Transport in blood: No transport protein- short plasma half-life
4-Mechanism of action:
Hormone binds to plasma membrane receptor
Hormone -receptor interaction leads to generation of second messenger
intracellularly that communicates (couples) hormones binding with
intracellular metabolic processes
Second messengers:
1- cyclic AMP (cAMP)
2- cyclic GMP (cGMP)
3- Calcium or phosphaitdyl inosiltol (or both)
4- Kinase cascade
Second Messenger: cAMP
List of Group II hormones
1- Second messenger is cAMP:
a2 & b adrenergic Catecholamines
Adrenocorticotropic Hormone (ACTH)
Angiotensin II
Antidiuretic Hormone (ADH)
Calcitonin
Human Chorionic Gonadotropins (hCG)
Corticotropin-releasing hormone (CRH)
Follicle Stimulating Hormone (FSH)
Leutinizing Hormone (LH)
Glucagon
Ipotropin (LPH)
MSH (Melanocyte Stimulating Hormone)
Parathyroid Hormone (PTH)
Somatostatin
Thyroid Stimulating Hormone (TSH)
List of Group II hormones
1- Second messenger is cGMP
Atrial natriuretic peptide (ANP)
Nitric Oxide (NO)
There are two isoforms of guanylate cyclase involved in production of cGMP:
• A membrane-bound guanylate cyclase ( for ANP & bacterial endotoxin)
• A cytosolic guanylate cyclase (for NO)
Second messenger cGMP:
Atrial Natriuretic Peptide
NO• signaling in physiology
Nitric Oxide Synthase
O2-•
NO•
ONOO-
Binds to heme moiety of
guanylate cyclase
Conformational change
of the enzyme
Increased activity
(production of cGMP)
Modulation of activity of
other proteins (protein
kinases, phosphodiesterases, ion
channels)
Physiological response
(relaxation of smooth
muscles, inhibition of
platelet aggregation,
etc.)
Second Messenger: Phosphatidyl inositol & calcium
List of Group II hormones
Second messenger is phosphatidyl inositol or calcium (or
both together)
Acetylcholine
a1 adrenergic catecholamines
Angiotensin II
Antideuretic Hormone (ADH)
Cholecystokinin
Gastrin
Gonadotropin –Releasing Hormone (GnRH)
Oxytocin
Platelet Derived Growth Factor (PDGF)
Substance P
Thyrotropin Releasing Hormone (TRH)
Second Messenger: Kinase cascade
(for insulin hormone)
Binding
of insulin on a-subunits
Of insulin receptors
Phosphorylation
of b-subunits
Of insulin receptors
Phosphorylation
of insulin response substrates (IRS)
CHANGE
IN
Gene
Expression
Metabolism
Growth
List of Group II hormones
Second messenger is kinase cascade:
Chorionic somatomammotropin (CS)
Epidermal Growth factor (EGF)
Erythropoeitin (EPO)
Antideuretic Hormone (ADH)
Fibroblast Growth Factors (FGF)
Growth Hormone (GH)
Insulin
Insulin-like Growth factors (IGF-I & IGF-II)
Nerve Growth Factor (NGF)
Platelet Derived Growth Factor (PDGF)
Prolactin (PRL)