Transcript Alcyomics Ltd - In

Skimune™ Technologies
for sensitization and potency
assessment
Unique tools for the Cosmetic and Chemical Industries.
www.alcyomics.com
Skin Explant Technology
Blood Donor
Immature Mo-DC´s
- Response
Skimune TM technology
Mature DC´s + compound
+ T Cells
Sensitized T cells +
Skin biopsy
+ Response
Histopathological evaluation
Grade I
Grade II
Grade III
Grade IV
in vitro tissue damage can predict in vivo tissue damage, tissue damage graded I-IV
(Lerner, K.G., et al., Transplant Proc, 1974. 6(4): p. 367-7)
Grade 0 = normal skin
Grade I = mild vacuolization of epidermal cells
Grade II = diffuse vacuolization of basal cells with scattered dyskeratotic bodies
Grade III = sub-epidermal cleft formation
Grade IV = complete epidermal separation
SkimuneTM assay
What it models:
Damaged skin
The sensitization and elicitation phases of the skin sensitization process. Adapted from (http://www.kao.com/jp/en/corp_rd/skills_01_04.html))
The SkimuneTM model models the in vivo acute contact dermatitis (ACD) model
Assay evaluation
SkimuneTM
–
LLNA
The SkimuneTM assay was evaluated against the mouse local lymph node
assay (LLNA), which is the European gold standard for sensitization testing
Number of positive LLNA chemicals = 20
SkimuneTM prediction of positive chemicals = 19
Number of negative LLNA chemicals = 20
SkimuneTM prediction of negative chemicals = 19
95% specificity and 95% sensitivity, concordance 95%
SkimuneTM technologies detect human reactions
Reactions to Nickel Sulphate ( NiSO4) cannot be picked up using mouse models
of toxicology-mouse physiology is not human physiology !!
Potency assessment
Potency assessment of
cinnamic alcohol and
oxazalone.
Skin explant results show
histopathological damage in skin
in response to 0.1µM, 0.05µM
and 0.01µM concentration of
each chemical. For Cinnamic
alcohol 0.1µM gave a grade III
positive response, 0.05µM and
0.01µM gave a grade I negative
response. Oxazalone gave a grade
III positive response at 0.1µM and
0.05µM and a grade I negative
response at 0.01µM. Results show
oxazalone is a more potent
chemical than cinnamic alcohol
Case study 1- Cosmeceuticals
Determine the effect of repeated applications of product X in skin
Result:
Positive control
Product X Day3
Product X Day2
Negative control
Confidential
Repeated application of
Product X over a 3 day period
showed an increase in damage.
Results show a grade II/III
positive response on day 2 and
a grade III positive response
on day 3.
Results indicate that product
X has the potential to cause
an adverse response.
Case study 2- Cosmeceuticals
Determine in-vitro responses to wheat protein products
Medium Grade I
Compound 1 Grade I
Compound 2Grade III
Two wheat protein products were tested for immunogenic responses. Compound 2
gave a positive response showing a high degree of histological damage indicating
that is likely to cause an adverse reaction. Compound 1 showed no damage
response in skin histology
www.alcyomics.com
Contact: [email protected]
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